Antibody Array for Protein Expression
Antibody Array is a high-throughput ELISA based platform for efficient protein expression profiling in cells, tissues, serum, or even culture media. Compared to the conventional methods such as Western Blots or traditional ELISA, antibody array assays are much more productive and cost-effective.
Our broad exploratory antibody arrays, including Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array, allow investigators to examine up to 1358 proteins on a single slide. The smaller pathway focused arrays are designed for researchers to study highly relevant proteins in their specific research fields.
We offers a wide range of phospho antibody arrays for protein phosphorylation profiling. The antibodies on our phospho arrays are site-specific and phospho-specific, allowing investigators to study tyrosine, serine, and threonine phosphorylation at specific sites.
Antibodies on our antibody arrays are covalently immobilized on high quality glass surface coated with proprietary 3-D polymer materials to ensure high binding efficiency and specificity. Each array includes well-characterized antibodies, positive controls, and negative controls. To maximize data reliability, each antibody is printed with two or six replicates.
- Qualitative protein expression profiling
- Compare profiles of normal, diseased or treated samples
- Measure phosphorylation changes at specific sites (Phospho Antibody Arrays)
- Identify candidate biomarkers
How Antibody Array Works
The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated streptavidin.
The antibody array platform utilizes sensitive fluorescent detection. The arrays can be scanned on most microarray scanners compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides. Here is a list of commonly used compatible array scanners.
Size: 2 Slides/Pack for processing two unique samples
Slide dimensions: 76 x 25 x 1 mm
Storage Condition : 4°C for 6 months
Shipping Condition: 4°C with ice packs
Protein Expression Profiling Arrays
|Product Name||Catalog No.||Price (2 slides/pk)|
|Apoptosis Antibody Array||APP069||$360|
|Cancer BioMarker Array||SCB200||$730|
|Cell Cycle Antibody Array||ACC058||$320|
|Cytokine Profiling Array||SCK100||$840|
|Explorer Antibody Array||ASB600||$840|
|Kinase Antibody Array||AVK276||$730|
|MAPK Signaling Array||SMK060||$330|
|Signal Transduction Array||AST160||$550|
|Signaling Explorer Array||SET100||$1,450|
Phosphorylation Profiling Arrays
Selected Recent Publications
Signaling Explorer Array: Garnett D, Caveolae as a target to quench autoinduction of the metastatic phenotype in lung cancer, J Cancer Res Clin Oncol 2016 142:611–618
Phospho Explorer Array: Hillard T, Miklossy G, 15∝-methoxypuupehenol induces antitumor effects in vitro and in vivo against human glioblastoma and breast cancer models, Mol Cancer Ther, 2017 Jan 9, doi: 10.1158/1535-7163
Cell Cycle Control Phospho Antibody Array: Lee S, Jang J, Latent Kaposi’s sarcoma-associated herpesvirus infection in bladder cancer cells promotes drug resistance by reducing reactive oxygen species, J Microbiol 2016 Nov, 4(11):782-788
Cytokine Profiling Array: Luo MXM, Wong SH, Autophagy Mediates HBx-Induced Nuclear Factor-κB Activation and Release of IL-6, IL-8 and CXCL2 in Hepatocytes, Journal of Cellular Physiology, 2015, DOI: 10.1002/jcp.24967