Cell Cycle Control Phospho Antibody Array

Cell Cycle Control Phospho Antibody Array

Cell Cycle Control Phospho Antibody Array features 238 highly specific antibodies important to the cell cycle control system and DNA damage/repair mechanism. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PCC238

$660.00

Cell Cycle Control Phospho Antibody Array

Cell Cycle Control Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It’s designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine, serine, and threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening
  • Suitable samples: cell lysates; frozen or FFPE tissue lysates
  • Antibodies are covalently attached to 3D polymer coated glass slides
  • High specificity and affinity; low background
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 238 site-specific and phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 85%; Rat: 57%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months

 

Recent Publications

Gruosso T, Mieulet V, Chronic oxidative stress promotes H2AX protein degradation and enhances chemosensitivity in breast cancer patientsEMBO Mol Med 2016 Mar 22. pii: e201505891. doi: 10.15252/emmm.201505891

Lee S, Jang J, Latent Kaposi’s sarcoma-associated herpesvirus infection in bladder cancer cells promotes drug resistance by reducing reactive oxygen speciesJ Microbiol 2016 Nov, 4(11):782-788

Ordering Information
Catalog No Description Size Price
PCC238 Cell Cycle Control Phospho Antibody Array 2 slides/pk $660
Additional Reagent Needed:

The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

Antibody Array - How It Works

 Questions? Visit our Frequently Asked Questions page.

14-3-3 theta/tau (Ser232), 14-3-3 zeta (Ser58), 14-3-3 zeta/delta (Thr232), Abl1 (Thr754/735), Abl1 (Tyr204), Abl1 (Tyr412), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT2 (Ser474), BRCA1 (Ser1423), BRCA1 (Ser1457), BRCA1 (Ser1524), c-Abl (Tyr245), c-Abl (Tyr412), CDC2 (Tyr15), CDC25A (Ser124), CDC25A (Ser75), CDC25B (Ser323), CDC25B (Ser353), CDC25C (Ser216), CDC25C (Thr48), CDK1/CDC2 (Thr14), CDK2 (Thr160), CDK7 (Thr170), Chk1 (Ser280), Chk1 (Ser286), Chk1 (Ser296), Chk1 (Ser301), Chk1 (Ser317), Chk1 (Ser345), Chk2 (Ser516), Chk2 (Thr383), Chk2 (Thr387), Chk2 (Thr68), Cyclin A (A1/A2), Cyclin A1, Cyclin B1 (Ser126), Cyclin B1 (Ser147), Cyclin D1 (Thr286), Cyclin D3 (Thr283), Cyclin E1 (Thr395), Cyclin E1 (Thr77), DNA-PK (Thr2638), DNA-PK (Thr2647), E2F1 (Thr433), E2F2, E2F4, E2F6, FKHR (Ser256), FKHR (Ser319), FKHRL1 (Ser253), FOXO1/3/4-PAN (Thr24/32), FOXO1A (Ser329), FOXO1A/3A (Ser322/325), GSK3a-b (Tyr216/279), GSK3beta (Ser9), HDAC1 (Ser421), HDAC2 (Ser394), HDAC3 (Ser424), HDAC4 (Ser632), HDAC5 (Ser259), HDAC5 (Ser498), HDAC6 (Ser22), HDAC7, HDAC8 (Ser39), HDAC9, HDAC10, Histone H2A.X (Ser139), MDM2 (Ser166), MDM4 (Ser367), Myc (Ser373), Myc (Ser62), Myc (Thr358), Myc (Thr58), p15INK, p18INK, p21Cip1 (Thr145), p27Kip1 (Ser10), p27Kip1 (Thr187), p300, p300/CBP, p53 (Ser6), p53 (Ser9), p53 (Ser20), p53 (Ser15), p53 (Ser33), p53 (Ser37), p53 (Ser46), p53 (Ser315), p53 (Ser366), p53 (Ser378), p53 (Ser392), p53 (Thr18), p53 (Thr81), p90RSK (Ab-359/363), p90RSK (Ser380), p90RSK (Thr359/Ser363), p90RSK (Thr573), p95/NBS1 (Ser343), PP2A-a (Tyr307), RAD51 (Tyr315), RAD52 (Tyr104), Rb (Ser608), Rb (Ser780), Rb (Ser795), Rb (Ser807), Rb (Ser811), Rb (Thr821), Smad2/3 (Thr8), Smad3 (Ser204), Smad3 (Ser208), Smad3 (Ser213), Smad3 (Ser425), Smad3 (Thr179), Smad4, TGFB1, TGFB2, TGFB3, TGFGBR2, TOP2A (Ser1106), WEE1 (Ser642)

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

Elias D, Vever H, Gene expression profiling identifies FYN as an important molecule in tamoxifen resistance and a predictor of early recurrence in patients treated with endocrine therapy, Oncogene 2014, 1-9

Gruosso T, Mieulet V, Chronic oxidative stress promotes H2AX protein degradation and enhances chemosensitivity in breast cancer patientsEMBO Mol Med 2016 Mar 22. pii: e201505891. doi: 10.15252/emmm.201505891

Lee S, Jang J, Latent Kaposi’s sarcoma-associated herpesvirus infection in bladder cancer cells promotes drug resistance by reducing reactive oxygen speciesJ Microbiol 2016 Nov, 4(11):782-788

Ma J, Sun F, Depletion of intermediate filament protein Nestin, a target of microRNA-940, suppresses tumorigenesis by inducing spontaneous DNA damage accumulation in human nasopharyngeal carcinoma, Cell Death and Disease 2014, 5:e1377.

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,350 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $250 per slide

 

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