Cell Signaling Explorer Antibody Array

Cell Signaling Explorer Antibody Array

Cell Signaling Explorer Antibody Array features 1358 unique antibodies covering 20 cellular signaling pathways, ideal for high-throughput biomarker screening and discovery. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: SET100

$1,450.00

Cell Signaling Explorer Antibody Array

The Cell Signaling Antibody Array is a high-throughput ELISA based antibody array for qualitative/semi-quantitative protein expression profiling. The array is designed for broad screening of protein level changes in multiple cellular signaling pathways, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.

Key Features
  • Qualitative/semi-quantitative protein expression profiling
  • Suitable samples: cell lysates; frozen or FFPE tissue lysates; serum; culture media
  • Glass based array with high specificity and low background
  • Antibodies are covalently attached to 3D polymer coated glass slides
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 1358 antibodies
Number of Replicates: 2 replicates per antibody
Reactivity: Human
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months

 

Selected Publications

Garnett D, Caveolae as a target to quench autoinduction of the metastatic phenotype in lung cancer, J Cancer Res Clin Onco 2016 142:611–618

Klimushina MV, Gumanova NG, Direct labeling of serum proteins by fluorescent dye for antibody microarray, Biochem Biophys Res Commun 2017; 486(3):824-826

Park HJ, Oh JS, Proton Irradiation Sensitizes Radioresistant Non-small Cell Lung Cancer Cells by Modulating Epidermal Growth Factor Receptor-mediated DNA Repair, Anticancer Res, 2016 36(1):205-12

Ordering Information
Catalog No Description Size Price
SET100 Signaling Explorer Antibody Array 2 slides/pk $1450
Additional Reagent Needed:

The ELISA based Cell Signaling Explorer Antibody Array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

Signaling Antibody Array - How It Works

Questions? Visit our Frequently Asked Questions page.

14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 theta, 14-3-3 zeta, 4E-BP1, 5-HT-1A, 5-HT-1F, 5-HT-2C, 5-HT-3A, 5-HT-4, 5-HT-5A, A1BG, A26C2/3, AARSD1, AASDHPPT, AATF, ABCA8, ABCB7, ABCD1, ABHD11, ABHD12, ABHD12B, ABHD14A, ABHD14B, ABHD4, ABL1, ACAD10, ACBD6, ACOT2, ACOT4, ACSL6, Actin-alpha-1, Actin-gamma2, Actin-pan, ACTL6A, ACTN 1/2/3/4, ACTN alpha-2/3, ACTR-1C, ACTR3, ACVL1, ADA2L, ADAM 17, ADAR1, ADCK1, ADCK2, ADCK3, ADCK5, ADCY4, ADCY5/6, ADCY7, ADCY8, ADD2, ADD3, ADH7, ADK, ADNP, ADPGK, ADRB1, Adrenergic Receptor alpha-2A, Adrenergic Receptor alpha-2B, Adrenergic Receptor alpha-2C, Aggrecan, AGR3, and more

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

Garnett D, Caveolae as a target to quench autoinduction of the metastatic phenotype in lung cancer, J Cancer Res Clin Oncol 2016 142:611–618

Klimushina MV, Gumanova NG, Direct labeling of serum proteins by fluorescent dye for antibody microarray, Biochem Biophys Res Commun 2017; 486(3):824-826

Lund R, Leth-Larsen R, NADH-cytochrome b5 reductase 3 promotes colonization and metastasis formation and is a prognostic marker of disease-free and overall survival in estrogen receptor-negative breast cancer, Mol Cell Proteomics 2015 14(11):2988-99

Park HJ, Oh JS, Proton Irradiation Sensitizes Radioresistant Non-small Cell Lung Cancer Cells by Modulating Epidermal Growth Factor Receptor-mediated DNA Repair, Anticancer Res 2016 36(1):205-12 

Wu J, Qian J, Abstract 5547: Exploration of selective small molecule compounds as caspase inhibitors for treatment of oral mucositis caused by cancer therapy, [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013 73(8 Suppl):Abstract nr” 5547

Additional publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $2,050 per sample

Array Scanning & Image Analysis Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning (free of charge) and analysis.

 

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