TGF-beta Phospho Antibody Array

TGF-beta Phospho Antibody Array

TGF-β (TGF-beta) Phospho Antibody Array features 176 antibodies linked to the TGF-beta signaling pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PTG176

$560.00

TGF-beta Phospho Antibody Array 

Transforming growth factor beta (TGF-β) signaling plays an important role in cell proliferation, differentiation, and repair of many organisms. The signaling process begins with a dimeric TGF-β superfamily ligand binding to a Type II receptor, which enlists a Type I receptor (TGFBR1) and phosphorylates its serine residues. The activated TGFBR1 then phosphorylates and activates cytoplasmic molecules Smad2 and Smad3 for the TGF-β pathway or Smad1/5/9 for the bone morphogenetic protein (BMP) pathway. The phosphorylation of Smads leads to formation of a hetero-oligomeric complex with Smad4, which then moves into the nucleus and interacts with transcription promoters/cofactors and regulates gene expression. TGF-β also participates in the regulation of non-Smad pathways including ERK1/2, JNK/SAPK, and p38 MAP kinase pathways.

TGF-β (TGF-beta) Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to investigate serine/threonine and tyrosine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening
  • Suitable samples include: cell lysates; frozen or FFPE tissue lysates
  • Glass based array with high specificity and low background
  • Antibodies covalently attached to 3D polymer coated glass surface
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 176 site-specific and phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 97%; Rat: 87%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months

 

Specifications

Size: 2 Slides/Pack
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Barcode label: 23 x 9 mm
Spot-to-spot distance: 600 um
Storage Condition : 4°C for 6 months

Recent Publications

Guo J, Wang Q, Visible red and infrared light alters gene expression in human marrow stromal fibroblast cellsOrthodontics & Craniofacial Research 2015; 18(Suppl.1): 5061

Hasan M, Neumann B, Activation of TGF-β-induced non-Smad signaling pathways during Th17 differentiation, Immunology and Cell Biology, 2015 Aug;93(7):662-72

Wu Y, Su M, Abnormal expression of TGF-beta type II receptor isoforms contributes to acute myeloid leukemia, Oncotarget 2017, 8(6):10037-10049

Ordering Information
Catalog No Description Size Price
PTG176 TGF-beta Phospho Antibody Array 2 slides/pk $560
Additional Reagent Needed:

The ELISA based TGF-beta (TGF-β) antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

TGF-beta Antibody Array - How It Works

 Questions? Visit our Frequently Asked Questions page.

AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT2 (Ser474), c-Abl (Tyr245), c-Abl (Tyr412), cofilin (Ser3), ERK1 (Thr202), ERK1 (Tyr204), ERK1/2, ERK3 (Ser189), ERK8 (Thr175/Tyr177), Gab2 (Tyr643), JNK1/2/3 (Thr183/Tyr185), JNKK, LIMK1 (Thr508), MAP3K1 (Thr1381), MKK3 (Ser189), MKK3 (Thr222), MKK6 (Ser207), mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), Myc (Ser373), Myc (Ser62), Myc (Thr358), Myc (Thr58), p38 MAPK (Thr180), p38 MAPK (Tyr182), p38 MAPK (Tyr322), PAK1 (Ser204), PAK1 (Thr212), PAK1/2 (Ser199), PAK1/2/3 (Ser141), PAK1/2/3 (Thr423/402/421), PAK2 (Ser192), PAK2 (Ser20), PAK3 (Ser154), PI3K-p85-a (Tyr607), PI3K-p85-a/g (Tyr467/199), PKC alpha (Tyr657), PKC alpha/beta II (Thr638), PKC beta (Ser661), PKC delta (Ser645), PKC delta (Thr505), PKC epsilon (Ser729), PKC theta (Ser676), PKC theta (Thr538), PKC zeta (Thr410), PKC zeta (Thr560), PP2A-alpha (Tyr307), Rac1/cdc42 (Ser71), Ras-GRF1 (Ser916), Rho/Rac GEF2 (Ser885), S6 Ribosomal Protein (Ser235), S6K, SAPK (Thr183), SAPK (Tyr185), SEK1 (Ser80), SEK1 (Thr261), Shc (Tyr349), Shc (Tyr427), Smad1 (Ser187), Smad1 (Ser465), Smad1/5/9, Smad2 (Ser250), Smad2 (Ser467), Smad2 (Thr220), Smad2/3 (Thr8), Smad3 (Ser204), Smad3 (Ser208), Smad3 (Ser213), Smad3 (Ser425), Smad3 (Thr179), Smad4, SP1 (Thr739), TAK1 (Thr184), TGF alpha, TGFB1, TGFB2, TGFB3, TGFBR2

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

Assayag M, Berkman N, EDA-Fibronectin Modulates The Behaviour Of Airway Fibroblasts From Chronic Ovalbumin Challenged MiceD29. Molecular Signals and Cellular Mechanics: Focus On Asthma. May 1, 2014, A5602-A5602

Gerbaud P, Pidoux G, Mesenchymal Activin-A Overcomes Defective Human Trisomy 21 Trophoblast Fusion, Endocrinology 2011, Published online before print September 27, 2011, doi: 10.1210/en.2011-1193

Guo J, Wang Q, Visible red and infrared light alters gene expression in human marrow stromal fibroblast cellsOrthodontics & Craniofacial Research 2015; 18(Suppl.1): 5061

Han B, Luo H, Raelson J, TGFBI (βIG-H3) is a diabetes risk gene based on mouse and human genetic studies, Oxford Journals of Human Molecular Genetics 2014 April, 149-173

Hasan M, Neumann B, Activation of TGF-β-induced non-Smad signaling pathways during Th17 differentiation, Immunology and Cell Biology, 2015 Aug;93(7):662-72

Iwata, J, Suzuki, A, Non-canonical transforming growth factor beta (TGFb) signaling in cranial neural crest cells causes tongue muscle developmental defects, Journal of Biological Chemistry 2013, doi:10.1074/jbc.M113.493551jbc.M113.493551

Wu Y, Su M, Abnormal expression of TGF-beta type II receptor isoforms contributes to acute myeloid leukemia, Oncotarget 2017, 8(6):10037-10049

Zhang Y, Li Q, GDF11 improves tubular regeneration after acute kidney injury in elderly mice, Scientific Reports 6, Article number: 34624

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,300 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $200 per slide

 

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