Cancer Signaling Phospho Antibody Array

Cancer Signaling Phospho Antibody Array

The newly updated Cancer Signaling Antibody Array features 269 highly specific antibodies important to cancer signaling pathways. The array is designed for protein phosphorylation profiling in cancer cell and tissue samples from human, mouse, or rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PCS248

$690.00

Cancer Signaling Antibody Array

The Cancer Signaling Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling, designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies for studying serine, threonine, and tyrosine phosphorylation specific sites. The 269 antibodies on this array allow researchers to examine key proteins from multiple cancer cellular pathways including AKT signaling, apoptosis, EGF/EGFR signaling, ERK/MAPK, p53 signaling and more.

Key Features
  • Site-specific phosphorylation profiling and screening
  • Suitable samples: cell lysates; frozen or FFPE tissue lysates
  • Glass based array with high specificity and low background
  • Antibodies are covalently attached to 3D polymer coated glass slides
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 269 site-specific and phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 88%; Rat: 68%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months

 

Recent Publications

Ling S, Xie H, Metformin potentiates the effect of arsenic trioxide suppressing intrahepatic cholangiocarcinoma: roles of p38 MAPK, ERK3, and mTORC1, J Hematol Oncol, 2017 10(1):59

Oda K, Umemura M, Transient receptor potential cation 3 channel regulates melanoma proliferation and migration, J Physiol Sci, 2016 Sep 9, DOI: 10.1007/s12576-016-0480-1

Nekova T, Kneitz S, Silencing of CDK2, but not CDK1, separates mitogenic from anti-apoptotic signaling, sensitizing p53 defective cells for synthetic lethality, Cell Cycle, 2016 (23):3203-3209

Ordering Information
Catalog No Description Size Price
PCS248 Cancer Signaling Phospho Antibody Array 2 slides/pk $690
Additional Reagent Needed:

The ELISA based Cancer Signaling Phospho Antibody Array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

Antibody Array Assay Principle

  Questions? Visit our Frequently Asked Questions page.

 

14-3-3 zeta (Ser58), 4E-BP1 (Thr36), AKT (Ser473), AKT (Thr308), AKT2 (Ser474), AMPK1 (Thr174), BAD (Ser112), BAD (Ser136), BAD (Ser155), BCL2 (Ser70), BCL2 (Thr56), BCL-XL (Ser62), BRCA1 (Ser1423), BRCA1 (Ser1524), CaMK2 (Thr286), Catenin b (Ser33), Catenin b (Ser37), Catenin b (Thr41/Ser45), Caspase 3 (Ser150), Caspase 9 (Ser144), Caspase 9 (Thr125), Caspase 9 (Tyr153),Caveolin-1 (Tyr14), CDC2 (Tyr15), CDC25A (Ser75), CDC25C (Ser216), CDK2 (Thr160), Chk1 (Ser280), Chk1 (Ser317), Chk1 (Ser345), Chk2 (Ser516), Chk2 (Thr68), c-Jun (Thr239), c-Jun (Ser243), c-Jun (Ser73), c-Kit (Tyr721), CREB (Ser133), CrkII (Tyr221), eEF2K (Ser366), EGFR (Tyr1110), eIF2A (Ser51), eIF4E (Ser209), Elk1 (Ser383), ERK3 (Ser189), ERK8 (Thr175/Tyr177), Estrogen Receptor-a (Ser167), FAK (Tyr397), FAK (Tyr861), FAK (Tyr925), FGFR1 (Tyr154), FKHR (Ser256), GSK3 alpha (Ser21), GSK3 beta (Ser9), HDAC8 (Ser39), HER2 (Tyr877), Histone H2A.X (Ser139), HSF1 (Ser303), HSP27 (Ser15), HSP27 (Ser78), HSP90B (Ser254), ICAM-1 (Tyr512), IGF-1R (Tyr1161), IkB-alpha (Ser32/36), IkB-alpha (Tyr42), IkB-beta (Ser23), IkB-epsilon (Ser22), IKK-alpha (Thr23), Integrin beta-3 (Tyr773), Integrin beta-3 (Tyr785), JAK1 (Tyr1022), JAK2 (Tyr1007), JAK2 (Tyr221), JunB (Ser259), JunB (Ser79), JunD (Ser255), Keratin 18 (Ser33), MEK1 (Ser217), MEK1 (Ser221), MEK1 (Thr291), MEK-2 (Thr394), Met (Tyr1349), MKK3 (Ser189), MSK1 (Ser376), mTOR (Ser2448), Myc (Ser62), Myc (Ser373), Myc (Thr358), Myc (Thr58), NFkB-p100/p52 (Ser865), NFkB-p100/p52 (Ser869), NFkB-p105/p50 (Ser893), NFkB-p105/p50 (Ser907), NFkB-p105/p50 (Ser932), NFkB-p65 (Ser529), NFkB-p65 (Thr254), p21Cip1 (Thr145), p27Kip1 (Ser10), p27Kip1 (Thr187), P38 MAPK (Thr180), P38 MAPK (Tyr182), p44/42 MAPK (Thr202), p44/42 MAPK (Tyr204), p53 (Ser6), p53 (Ser315), P70S6K (Ser424), PDGF R beta (Tyr751), PDK1 (Ser241), PI3K p85a (Tyr607), PTEN (Ser380/Thr382/Thr383), Pyk2 (Tyr402), Rac1/cdc42 (Ser71), Raf1 (Ser259), Rb (Ser780), Rel (Ser503), SAPK/JNK (Thr183), Shc (Tyr349), SHP-2 (Tyr580), Smad3 (Ser425), Src (Tyr418), Src (Tyr529), STAT1 (Ser727), STAT1 (Tyr701), STAT3 (Ser727), STAT3 (Tyr705), STAT4 (Tyr693), STAT5A (Ser780), STAT5A (Tyr694), STAT6 (Thr645), STAT6 (Tyr641), Tau (Ser404), Trk B (Tyr515), TYK2 (Tyr1054), VEGFR2 (Tyr951)

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

Bhola NE, Thomas SM, Targeting GPCR-mediated p70S6K activity may improve head and neck cancer response to cetuximab, Clinical Cancer Research 2011, 17(15):4996-5004

Gu C, Feng M, RalA, a GTPase targeted by miR‑181a, promotes transformation and progression by activating the Ras‑related signaling pathway in chronic myelogenous leukemia, Oncotarget, 2016 Mar 8. doi: 10.18632/oncotarget.7987

El-Haibi CP, Singh R, Antibody Microarray Analysis of Signaling Networks Regulated by Cxcl13 and Cxcr5 in Prostate Cancer, Journal of Proteomics and Bioinformatics 2012, 5(8): 177-184

Ling S, Xie H, Metformin potentiates the effect of arsenic trioxide suppressing intrahepatic cholangiocarcinoma: roles of p38 MAPK, ERK3, and mTORC1, J Hematol Oncol, 2017 10(1):59

Nekova T, Kneitz S, Silencing of CDK2, but not CDK1, separates mitogenic from anti-apoptotic signaling, sensitizing p53 defective cells for synthetic lethality, Cell Cycle 2016 (23):3203-3209

Nekova TS, Kneitz S, Silencing of Dicer1 temporally separates pro- and anti-apoptotic signaling and confers susceptibility to chemotherapy in p53 mutated cells, Cell Cycle 2014 Jul 15;13(14):2192-8

Oda K, Umemura M, Transient receptor potential cation 3 channel regulates melanoma proliferation and migration, J Physiol Sci, 2016 Sep 9, DOI: 10.1007/s12576-016-0480-1

Watanabe T, Takahashi A, Epithelial–mesenchymal transition in human gastric cancer cell lines induced by TNF-α-inducing protein of Helicobacter pylori, International Journal of Cancer 2014 134(10):2373-82. doi: 10.1002/ijc.28582

Zhu R, Wang Q, pH sensitive nano layered double hydroxides reduce the hematotoxicity and enhance the anticancer efficacy of etoposide on non-small cell lung cancer, Acta Biomaterialia, 2016 29:320-332

Zhu Y, Dai B, Long non-coding RNA LOC572558 inhibits bladder cancer cell proliferation and tumor growth by regulating the AKT-MDM2-p53 signaling axis, Cancer Lett, 2016 Apr 26, doi:10.1016/j.canlet.2016.04.030

Zhu Y, Wan F, Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth, Oncotarget 2015 Jun 10; 6(16): 14488–14496

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,350 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $250 per slide

 

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