AMPK Signaling Phospho Antibody Array

AMPK Signaling Phospho Antibody Array

AMPK Phospho Antibody Array features 174 highly specific antibodies important to the AMPK (AMP-activated protein kinase) signaling pathway. The array is designed for protein phosphorylation profiling protein in cell and tissue samples from human, mouse, or rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PAM174

$560.00

AMPK Signaling Phospho Antibody Array

AMPK Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. The array is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine phosphorylation and serine/threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening
  • Suitable samples include: cell lysates; frozen or FFPE tissue lysates
  • Glass based array with high specificity and low background
  • Antibodies covalently attached to 3D polymer coated glass surface
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 174 site-specific and phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 89%; Rat: 77%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months
Selected Publications

Chan O, Burke, JB, The chemokine CCL5 regulates glucose uptake and AMPK signaling in activated T cells to facilitate chemotaxisJournal of Biological Chemistry 2012, 287:29406-29416

Ordering Information 
Catalog No Description Size Price
PAM174 AMPK Signaling Phospho Antibody Array 2 slides/pk $560
Additional Reagent Needed:

The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

Antibody Array Assay - How it works

 Questions? Visit our Frequently Asked Questions page.

4E-BP1 (Ser65), 4E-BP1 (Thr36), 4E-BP1 (Thr45), 4E-BP1 (Thr70), ACC1 (Ser79), ACC1 (Ser80), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT1/2/3, AKT1S1 (Thr246), AKT2 (Ser474), AMPK1 (Thr174), AMPK1/AMPK2 (Ser485/491), AMPKbeta1 (Ser182), CaMK1-alpha (Thr177), CaMK1-beta, CaMK2 (Thr305), CaMK2 (Thr286), CaMK2-b/g/d (Thr287), CaMK4 (Thr196/200), CAMK5, Cyclin A1, Cyclin B1 (Ser126), Cyclin B1 (Ser147), EEF2 (Thr56), eEF2K (Ser366), eNOS (Ser1177), eNOS (Ser615), eNOS (Thr495), Fas ligand, HMG14, HMG20B, HMGB1, HMGB2, HNF4a (Ser304), HNF4a/g, HSF1 (Ser303), HSL (Ser552/563), HSL (Ser554), LKB1 (Ser428), LKB1 (Thr189), MEF2A (Ser408), MEF2A (Thr312), MEF2A (Thr319), MEF2B, MEF2C (Ser396), MEF2D (Ser444), mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), p21Cip1 (Thr145), p53 (Ser6), p53 (Ser9), p53 (Ser15), p53 (Ser20), p53 (Ser33), p53 (Ser37), p53 (Ser46), p53 (Ser315), p53 (Ser366), p53 (Ser378), p53 (Ser392), p53 (Thr18), p53 (Thr81), p70S6K (Ser371), p70S6K (Ser411), p70S6K (Ser418), p70S6K (Ser424), p70S6K (Thr229), p70S6K (Thr389), p70S6k (Thr421), p70S6K-beta (Ser423), PFKFB2 (Ser483), PI3K-p85-alpha (Tyr607), PI3K-p85-a/g (Tyr467/199), PKA CAT (Thr197), PKA-R2B (Ser113), PLC-beta (Ser1105), PLC-beta3 (Ser537), RapGEF1 (Tyr504), SREBP-1 (Ser439), Tuberin (Ser939), Tuberin (Thr1462)

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

Chan O, Burke, JB, The chemokine CCL5 regulates glucose uptake and AMPK signaling in activated T cells to facilitate chemotaxis, Journal of Biological Chemistry 2012, 287:29406-29416

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,300 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $200 per slide

 

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