AMPK Signaling Phospho Antibody Array

AMPK Signaling Phospho Antibody Array

AMPK Phospho Antibody Array features 174 highly specific antibodies important to the AMPK (AMP-activated protein kinase) signaling pathway. The array is designed for protein phosphorylation profiling protein in cell and tissue samples from human, mouse, or rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog No: PAM174

$650.00

AMPK Signaling Phospho Antibody Array

AMPK Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. The array is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine phosphorylation and serine/threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening with 174 antibodies
  • Antibodies covalently immobilized on 3D polymer coated glass slide
  • Fluorescent detection
Specifications
Product Size:2 array slides per package for two samples (untreated vs. treated)
Featured Antibodies:174 site-specific and phospho-specific antibodies
Reactivity:Human: 100% | Mouse: 89% | Rat: 77%
Suitable Sample Type:Cell lysate | Tissue lysate
Detection Method:Fluorescence | Compatible Scanners
Internal Controls:Positive controls: beta-actin | GAPDH | Negative controls
Slide dimensions: 76 x 25 x 1 mm
Storage Condition:4°C for 6 months
PRODUCT DETAILS

4E-BP1 (Ser65), 4E-BP1 (Thr36), 4E-BP1 (Thr45), 4E-BP1 (Thr70), ACC1 (Ser79), ACC1 (Ser80), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT1/2/3, AKT1S1 (Thr246), AKT2 (Ser474), AMPK1 (Thr174), AMPK1/AMPK2 (Ser485/491), AMPKbeta1 (Ser182), CaMK1-alpha (Thr177), CaMK1-beta, CaMK2 (Thr305), CaMK2 (Thr286), CaMK2-b/g/d (Thr287), CaMK4 (Thr196/200), CAMK5, Cyclin A1, Cyclin B1 (Ser126), Cyclin B1 (Ser147), EEF2 (Thr56), eEF2K (Ser366), eNOS (Ser1177), eNOS (Ser615), eNOS (Thr495), Fas ligand, HMG14, HMG20B, HMGB1, HMGB2, HNF4a (Ser304), HNF4a/g, HSF1 (Ser303), HSL (Ser552/563), HSL (Ser554), LKB1 (Ser428), LKB1 (Thr189), MEF2A (Ser408), MEF2A (Thr312), MEF2A (Thr319), MEF2B, MEF2C (Ser396), MEF2D (Ser444), mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), p21Cip1 (Thr145), p53 (Ser6), p53 (Ser9), p53 (Ser15), p53 (Ser20), p53 (Ser33), p53 (Ser37), p53 (Ser46), p53 (Ser315), p53 (Ser366), p53 (Ser378), p53 (Ser392), p53 (Thr18), p53 (Thr81), p70S6K (Ser371), p70S6K (Ser411), p70S6K (Ser418), p70S6K (Ser424), p70S6K (Thr229), p70S6K (Thr389), p70S6k (Thr421), p70S6K-beta (Ser423), PFKFB2 (Ser483), PI3K-p85-alpha (Tyr607), PI3K-p85-a/g (Tyr467/199), PKA CAT (Thr197), PKA-R2B (Ser113), PLC-beta (Ser1105), PLC-beta3 (Ser537), RapGEF1 (Tyr504), SREBP-1 (Ser439), Tuberin (Ser939), Tuberin (Thr1462)

The ELISA based AMPK Pathway Phospho Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Antibody Array - How It Works

Antibody Array User’s Guide

Antibody List

Array Map

GAL File  (To download, right click on the file name, then choose “Save target as”)

Material Safety Data Sheet (MSDS)

Frequently Asked Questions

ADDITIONAL SERVICES

If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.

Cost: Free

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $200 per slide

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,470 per sample

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