p53 Signaling Phospho Antibody Array

p53 Signaling Phospho Antibody Array

p53 Antibody Array features 196 highly specific antibodies related to the p53 signaling pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PFT196


p53 Signaling Phospho Antibody Array

p53 Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine phosphorylation and serine/threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening
  • Suitable samples include: cell lysates; frozen or FFPE tissue lysates
  • Glass based array with high specificity and low background
  • Antibodies covalently attached to 3D polymer coated glass surface
  • Sensitive fluorescent detection
User’s Guide  

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 196 site-specific and phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 84%; Rat: 61%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months


Selected Publications

Mazer J, Rosado A, The long non-coding RNA GAS5 differentially regulates cell cycle arrest and apoptosis through activation of BRCA1 and p53 in human neuroblastoma, Oncotarget 2017; 8(4):6589-6607

Ordering Information 
Catalog No Description Size Price
PFT196 p53 Signaling Phospho Antibody Array 2 slides/pk $600
Additional Reagent Needed:

The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated streptavidin.Antibody Array- How It Works

 Questions? Visit our Frequently Asked Questions page.

ATM, ATRIP(Ser68/72), Bax, BCL-2(Ser70), BCL-2(Ser87), BCL-2(Thr56), BCL-2(Thr69), BRCA1(Ser1423), BRCA1(Ser1457), BRCA1(Ser1524), Breast tumor kinase, Casp3(Ser150), CDK2(Thr160), Chk1(Ser280), Chk1(Ser286), Chk1(Ser296), Chk1(Ser301), Chk1(Ser317), Chk1(Ser345), Chk2(Ser516), Chk2(Thr383), Chk2(Thr387), Chk2(Thr68), c-Raf(Ser296), c-Raf(Ser43), Cyclin D1(Thr286), Cyclin D3(Thr283), Cyclin E1(Thr395), Cyclin E1(Thr77), DNA-PK(Thr2638), DNA-PK(Thr2647), E2F1(Thr433), E2F2, E2F4, E2F6, ERK1/2, ERK3(Ser189), ERK8(Thr175/Tyr177), HDAC1(Ser421), HDAC10, HDAC2 (Ser394), HDAC3(Ser424), HDAC4(Ser632), HDAC5(Ser259), HDAC5(Ser498), HDAC6(Ser22), HDAC7, HDAC8(Ser39), HDAC9, JNK1/2/3(Thr183/Tyr185), JNKK, MAP3K1/MEKK1(Thr1381), MAPKAPK2(Ser272), MAPKAPK2(Thr222), MAPKAPK2(Thr334), MDM2(Ser166), MEK1(Ser217), MEK1(Ser221), MEK1(Ser298), MEK1(Thr286), MEK1(Thr291), MEK2(Thr394), MEKKK1, MEKKK4, p21Cip1 (Ab-145), p21Cip1(Thr145), p300, p38 MAPK(Thr180), p38 MAPK(Tyr182), p38 MAPK(Tyr322), p44/42 MAPK(Thr202), p44/42 MAPK(Tyr204), p53(Ser15), p53(Ser20), p53(Ser315), p53(Ser33), p53(Ser366), p53(Ser37), p53(Ser378), p53(Ser392), p53(Ser46), p53(Ser6), p53(Ser9), p53(Thr18), p53(Thr81), PIAS1, PIAS2, PIAS3, PIAS4, PKC delta(Ser645), PKC delta(Thr505), PKC delta(Tyr313), PKC delta(Tyr52), PKC delta(Tyr64), Raf1(Ser621), Raf1(Ser259), Raf1 (Ser338), Raf1(Tyr341), Rb(Ser608), Rb(Ser780), Rb(Ser795), Rb(Ser807), Rb(Ser811), Rb(Thr821)

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)


Mazer J, Rosado A, The long non-coding RNA GAS5 differentially regulates cell cycle arrest and apoptosis through activation of BRCA1 and p53 in human neuroblastoma, Oncotarget 2017; 8(4):6589-6607

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,300 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $200 per slide


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