Phospho Explorer Antibody Array

Phospho Explorer Antibody Array

Phospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse and rat. This array consists of 1318 antibodies related to multiple signaling pathways and biological processes. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PEX100


Phospho Explorer Antibody Array

Phospho Explorer Antibody Array is a high-throughput ELISA based antibody array, designed for qualitative protein phosphorylation profiling, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This phospho antibody array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine and serine/threonine phosphorylation at specific sites.

  • Phosphorylation profiling with 1318 site-specific antibodies from over 30 signaling pathways
  • Suitable samples: cell lysates; frozen or FFPE tissue lysates
  • Glass based array with high specificity and low background
  • Antibodies are covalently attached to 3D polymer coated glass slides
  • Highly sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 1318 site-specific and phospho-specific antibodies
Number of Replicates: 2 replicates per antibody
Reactivity: Human: 99%; Mouse: 92%; Rat: 66%  
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months


Recent Publications

Bosma M, Gerling M, FNDC4 acts as an anti-inflammatory factor on macrophages and improves colitis in miceNat Commun 2016 7:11314

Hillard T, Miklossy G, 15∝-methoxypuupehenol induces antitumor effects in vitro and in vivo against human glioblastoma and breast cancer models, Mol Cancer Ther, 2017 Jan 9, doi: 10.1158/1535-7163

Jiang HL, Sun HF, SSBP1 Suppresses TGF-β-Driven Epithelial-to-Mesenchymal Transition and Metastasis in Triple-Negative Breast Cancer by Regulating Mitochondrial Retrograde SignalingCancer Res 2016 76(4):952-64

Shi F, Guo H, The PI3K inhibitor GDC-0941 enhances radiosensitization and reduces chemoresistance to temozolomide in GBM cell linesNeuroscience 2017, 346:298-308

Ordering Information 
Catalog No Description Size Price
PEX100 Phospho Explorer Antibody Array 2 slides/pk $1380
Additional Reagent Needed:

The ELISA based Phospho Explorer Antibody Array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

Antibody Array Assay - How It Works

 Questions? Check out our Frequently Asked Questions.


14-3-3 theta/tau (Ser232), 14-3-3 zeta (Ser58), 14-3-3 zeta/delta (Ab-232), 14-3-3 zeta/delta (Thr232), 4E-BP1 (Ser65), 4E-BP1 (Thr36), 4E-BP1 (Thr45), 4E-BP1 (Thr70), Abl1 (Tyr204), Abl1 (Tyr412), ACC1 (Ser79), ACC1 (Ser80), ACK1 (Tyr284), AFX (Ser197), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT2 (Ser474), ALK (Tyr1507), ALK (Tyr1604), AMPK1 (Thr174), Amyloid beta A4 (Thr743/668), Androgen Receptor (Ser213), Androgen Receptor (Ser650), A-RAF (Tyr301/302), Arrestin-1 (Ab-412), Arrestin-1 (Ser412), ASK1 (Ser83), ASK1 (Ser966), ATF-1 (Ser63), ATF2 (Ab-112/94), ATF2 (Ser112/94), ATF2 (Ser62/44), ATF2 (Thr69/51), ATF2 (Thr71/53), ATF2 (Thr73/55), ATF4 (Ser245), ATP1A1/Na+K+ ATPase1 (Ser23), ATPase (Ser16), ATP-Citrate Lyase (Ser454), ATRIP (Ser68/72), AurB (Thr232), AurB (Tyr12), Aurora Kinase (Thr288), AXL (Tyr691), and more

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File

pdf    Material Safety Data Sheet (MSDS)


Amaya CN, Mitchell DC, Rho kinase proteins display aberrant upregulation in vascular tumors and contribute to vascular tumor growth, BMC Cancer, 2017 Jul 14, 17(1):485. doi: 10.1186/s12885-017-3470-7

Arsenault RJ, Genovese KJ, Wild-type and mutant AvrA−Salmonella induce broadly similar immune pathways in the chicken ceca with key differences in signaling intermediates and inflammation, Poultry Science 2015 Nov 14. pii: pev344

Bazon D, Ng MR, Flow-induced HDAC1 phosphorylation and nuclear export in angiogenic sprouting, Sci Rep 2016, 6:34046

Bernier M, Paul RK, Negative Regulation of STAT3-mediated Cellular Respiration by SirT1, Journal of Biological Chemistry 2011, 286(22):19270-9

Bosma M, Gerling M, FNDC4 acts as an anti-inflammatory factor on macrophages and improves colitis in miceNat Commun 2016 7:11314

Cook DP, Adam RJ, CF airway smooth muscle transcriptome reveals a role for PYK2. JCI Insight. 2017;2(17)

Cui WY, Zhao S, Identification and characterization of Poly(I:C)-induced molecular responses attenuated by nicotine in mouse macrophages, Molecular Pharmacology 2013 Jan;83(1):61-72

Dai Z, Zhou S, Capn4 contributes to tumour growth and metastasis of hepatocellular carcinoma by activation of the FAK–Src signalling pathwaysThe Journal of Pathology 234.3 (2014): 316-328

Dong S, Jia C, The REG gamma Proteasome Regulates Hepatic Lipid Metabolism through Inhibition of Autophagy, Cell Metabolism 2013, 18(3), 380-391

Eke I, Hehlgans S, Comprehensive analysis of signal transduction in three-dimensional ECM-based tumor cell culturesJ Biol Methods 2015; 2(4): e31

Eke I, Schneider L, EGFR/JIP-4/JNK2 Signaling Attenuates Cetuximab-Mediated Radiosensitization of Squamous Cell Carcinoma Cells, Cancer Research 2012 73(1): 297-306

Eke I, Zscheppang K, Simultaneous β1 integrin-EGFR Targeting and Radiosensitization of Human Head and Neck CancerJournal of the National Cancer Institute (2015) 107(2): dju419doi: 10.1093/jnci/dju419

Gao Y, Bai X, Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesisNat Struct Mol Biol 2016 23(5):441-9

Gavine P, Wang M, Identification and validation of dysregulated MAPK7 (ERK5) as a novel oncogenic target in squamous cell lung and esophageal carcinomaBMC Cancer, 2015 15:454 DOI: 10.1186/s12885-015-1455-y

Ge Z, Diao H,Connexin 43 mediates changes in protein phosphorylation in HK-2 cells during chronic cadmium exposure. Environ Toxicol Pharmacol. 2017 Jul;53:184-190

Gutierrez CM, Lopez-Valdez R, A Breast Tissue Protein Expression Profile Contributing to Early Parity-Induced Protection Against Breast CancerCell Physiol Biochem 2015, 37(5):1671-1685

Han KH, Kim AK, Protein profiling and angiogenic effect of hypoxia-cultured human umbilical cord blood-derived mesenchymal stem cells in hindlimb ischemia, Tissue Cell, 2017,  pii: S0040-8166(16)30363-9

Havel LS, Kline ER, Vimentin regulates lung cancer cell adhesion through a VAV2–Rac1 pathway to control focal adhesion kinase activity, Oncogene 2014 May, 123

Hillard T, Miklossy G, 15∝-methoxypuupehenol induces antitumor effects in vitro and in vivo against human glioblastoma and breast cancer models, Mol Cancer Ther, 2017 Jan 9, doi: 10.1158/1535-7163

Hu Y, Pioli PD, EFEMP1 suppresses malignant glioma growth and exerts its action within the tumor extracellular compartment, Molecular Cancer 2011, 10:123

Huang M, Lou D, Pyrrolidine dithiocarbamate attenuates paraquat-induced acute pulmonary poisoning in vivo via transforming growth factor β1 and nuclear factor κB pathway interactionHum Exp Toxicol 2016 Feb 9, pii: 0960327116630351

Jia D, Jing Y, Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma, Cell Research 2014 (24): 204–217

Jiang HL, Sun HF, SSBP1 Suppresses TGF-β-Driven Epithelial-to-Mesenchymal Transition and Metastasis in Triple-Negative Breast Cancer by Regulating Mitochondrial Retrograde SignalingCancer Res 2016 76(4):952-64

Kang S, Elf S, p90 Ribosomal S6 Kinase 2 Promotes Invasion and Metastasis of Human Head and Neck Squamous Cell Carcinoma Cells, Journal of Clinical Investigation 2010, 120(4):1165-1177

Kogut M, Swaggerty CL, Critical role of glycogen synthase kinase-3β in regulating the avian heterophil response to Salmonella enterica serovar Enteritidis, Frontiers in Veterinary Science 2014, 1:10. doi:10.3389/fvets.2014.00010

Kogut MH, Swaggerty CL, Chicken-Specific Kinome Array Reveals that Salmonella enterica Serovar Enteritidis Modulates Host Immune Signaling Pathways in the Cecum to Establish a Persistence Infection, Int J Mol Sci, 2016 17(8):1207

Koh YJ, Park SC, inventors, Samsung Electronics Co., Ltd., assignee. Composition for stimulating neovascularization at a site in a subject comprising dapsone and use thereof. United States patent application 14/836503, filed Aug 26, 2015

Koppenhagen P, Dickreuter E, Head and neck cancer cell radiosensitization upon dual targeting of c-Abl and beta1-integrinRadiother Oncol. 2017 Jul;53:184-190

Kuang XY, Jiang HS, The phosphorylation-specific association of STMN1 with GRP78 promotes breast cancer metastasisCancer Lett 2016 377(1):87-96

Li L, Zhao D, REGγ deficiency promotes premature aging via the casein kinase 1 pathway, PNAS 2013, 110(27): 11005-11010

Li W, Zhang C, Withaferin A suppresses the up-regulation of acetyl-coA carboxylase 1 and skin tumor formation in a skin carcinogenesis mouse modelMolecular Carcinogenesis, 2015, doi: 10.1002/mc.22423

Luo L, Zhao L, Insulin-like growth factor binding protein-3 is a new predictor of radiosensitivity on esophageal squamous cell carcinomaSci Rep, 2015; 5: 17336

Mitchell SJ, The SIRT1 Activator SRT1720 Extends Lifespan and Improves Health of Mice Fed a Standard Diet, Cell Reports 6 2014 March, 836-843

Nold-Petry CA, Lo CY, IL-37 requires the receptors IL-18Rα and IL-1R8 (SIGIRR) to carry out its multifaceted anti-inflammatory program upon innate signal transduction, Nat Immunol, 2015 16(4):354-365

Park HL, Lee SM, IK acts as an immunoregulator of inflammatory arthritis by suppressing TH17 cell differentiation and macrophage activation, Sci Reports 7, 2017:40280

Pereira EM, Labilloy A, Characterization and Phosphoproteomic Analysis of a Human Immortalized Podocyte Model of Fabry Disease Generated Using the CRISPR/Cas9 Technology, Am J Physiol Renal Physiol, 2016 Sep 28:ajprenal.00283.2016. doi: 10.1152

Pulito C, Mori F, Cynara scolymus affects malignant pleural mesothelioma by promoting apoptosis and restraining invasionOncotarget, 2015 July 20;6(20):18134-50

Pulito C, Sacconi A, Antibody Array as a Tool for Screening of Natural Agents in Cancer Chemoprevention, Methods Mol Biol, 2016 1379:189-99

Shah A, Kannambath S, Calcineurin Orchestrates Lateral Transfer of Aspergillus fumigatus During Macrophage Cell Death, Am J Respir Crit Care Med, 2016 May 10

Shi F, Guo H, The PI3K inhibitor GDC-0941 enhances radiosensitization and reduces chemoresistance to temozolomide in GBM cell lines, Neuroscience 2017, 346:298-308

Shigyo M, Kuboyana T, Extracellular vimentin interacts with insulin-like growth factor 1 receptor to promote axonal growthScientific Reports 5, Article number: 12055 (2015), doi:10.1038/srep12055

Suh JS, Lee HJ, Control of cancer stem cell like population by intracellular target identification followed by the treatment with peptide-siRNA complex, Biochem & Biophys Res Commu, 2017 May 26,

Takahashi T, Uehara H, Inhibition of EP2/EP4 signaling abrogates IGF-1R-mediated cancer cell growth: Involvement of protein kinase C-θ activation, Oncotarget, 2015 6(7):4826-44

Tan X, Liu P, Phosphoproteome Analysis of Invasion and Metastasis-Related Factors in Pancreatic Cancer Cells, PLOS One, 2016 March 25, DOI: 10.1371/journal.pone.0152280

Totonchy J, Osborn J, Aberrant Proliferation in CXCR7+ Endothelial Cells via Degradation of the Retinoblastoma Protein, PLOS ONE 8(7): e69828. doi:10.1371/journal.pone.0069828

Trazzi S, Fuchs C, HDAC4: A Key Factor Underlying Brain Developmental Alterations in CDKL5 Disorder, Hum Mol Genet, 2016 July 27, 2016, pii: ddw231

Uckun FM, A rationally designed nanoparticle for RNA interference therapy in B-lineage lymphoid malignanciesEBioMedicine, Volume 1, Issues 2–3, December 2014, 101-102

Uckun, FM, Mitchell L, Development of Polypeptide-based Nanoparticles for Non-viral Delivery of CD22 RNA Trans-splicing Molecule as a New Precision Medicine Candidate Against B-lineage ALL, EBioMedicine, Volume 2, Issue 7, July 2015, 649–659

Vehlow A, Klapproth E, Adhesion- and stress-related adaptation of glioma radiochemoresistance is circumvented by β1 integrin/JNK co-targeting. Oncotarget. 2017;8(30):49224-49237

Xu J, Zhou L, The REGγ-proteasome forms a regulatory circuit with IκBε and NFκB in experimental colitis, Nat Commun, 2016 Feb 7:10761

Yang EJ, Mahmood U, Alterations in protein phosphorylation in the amygdala of the 5XFamilial Alzheimer’s disease animal model, J Pharmaco Sci, 2017;133(4):261-267

Yang Y, Lian S, Antibody Array Revealed PRL-3 Affects Protein Phosphorylation and Cytokine Secretion, PLoS One, 2017 Jan 9,

Yim JH, Yun JM, Estimation of low-dose radiation-responsive proteins in the absence of genomic instability in normal human fibroblast cells, Int J Radiat Biol, 2017 Jul 6:1-29. doi: 10.1080/09553002.2017.1350302

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $2,050 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $350 per slide



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