Cell Signaling Phospho Antibody Array

Cell Signaling Phospho Antibody Array

The Cell Signaling Phospho Antibody Array features 304 highly specific antibodies important to 16 commonly studied cell signaling pathways. The array is designed for protein phosphorylation profiling using cell and tissue samples from human, mouse, or rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog No: PCS300

$960.00

Cell Signaling Phospho Antibody Array

The Cell Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative profiling and screening of candidate biomarkers from 16 cell signaling pathways, including PI3K/AKT signaling, apoptosis, autophagy, cell cycle, ErbB, focal adhension, MAPK, p53 signaling, VEGF and more. It is designed to identify total protein changes and phosphorylation changes by comparing normal samples to treated/diseased samples. This array features site-specific and phospho-specific antibodies for studies of phosphorylation events at specific phospho sites.

Key Features
  • 304 antibodies from 16 cell signaling pathways
  • Site-specific phosphorylation profiling and screening
  • Antibodies covalently immobilized on 3D polymer coated glass slide
  • Fluorescent detection
Specifications
Product Size:2 array slides per package for analyzing two samples (untreated vs. treated)
Featured Antibodies:304 site-specific and phospho-specific antibodies; 6 replicates per antibody
Reactivity:Human: 99% | Mouse: 87% | Rat: 66%
Suitable Sample Type:Cell lysate | Tissue lysate
Detection Method:Fluorescence | Compatible Scanners
Internal Controls:beta-actin | GAPDH | Negative controls
Slide Dimensions: 76 x 25 x 1 mm
Storage Condition:4°C for 6 months

 

PRODUCT DETAILS

PI3K/AKT
AKT1, AKT2, BAD, BCL2, CASP9, CCND1, CCNE1, CDK2, CKDN1A, CHUK, CREB1, EGFR1, EIF4E, EIF4EBP1, ERBB2, FOXO3, GSK3B, HSP90AB1, IGF1R, IKBKB, IKBKG, IL4R, IRS1, ITGB3, JAK1, JAK2, KDR, MAP2K1, MDM2, MET, MTOR, MYC, NFKB1, NOS3, NTRK2, PDGFRB, PDK1, PPP2CA, PRKAA1, PTEN, PTK2, RAF1, RELA, RPS6KB1, STK11, SYK, TP53, TSC2, YWHAQ

AMPK
ACACA, AKT1, AKT1S1, AKT2, CCND1, CREB1, EEF2, EEF2K, EIF4EBP1, FOXO1, FOXO3, IFG1R, IRS1, MAP3K7, MTOR, PDPK1, PPARG, PPP2CA, PRKAA1, RPS6KB1, SREBF1, STK11, TSC2

Apoptosis
ACTB, ACTG1, AKT1, AKT2, BAD, BAX, BCL2, BID, CASP3, CASP8, CASP9, CHUK, EIF2S1, FADD, FOS, IKBKB, IKBKG, LMNA, MAP2K1, MAP3K5, MAPK9, NFKB1, NFKB1A, PDPK1, RAF1, RELA, TP53

Autophagy
AKT1, AKT1S1, AKT2, BAD, BCL2, BECN1, EIF2S1, IGF1R, IRS1, MAP2K1, MAP3K7, MAPK9, MTOR, PDPK1, PPP2CA, PRKAA1, PTEN, RAF1, RPS6KB1, STK11, TSC2

Cell Cycle
ABL1, CCND1, CCNE1, CDC25C, CDK2, CDKNIA, CHEK1, CHEK2, GSK3B, HDAC1, HDAC2, MDM2, MYC, PLK1, RB1, SMAD2, SMAD3, TP53, YWHAQ

ErbB
ABL1, AKT1, AKT2, BAD, BRAF, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CBL, CDKN1A, CRK, EGFR, EIF4EBP1, ELK1, ERBB2, GAB1, GSK3B, MAP2K1, MAP2K4, MAPK9, MTOR, MYC, PAK1, PLCG1, PTK2, RAF1, RPS6KB1, SHC1, SRC, STAT5A, STAT5B ,

Focal Adhesion
ACTB, ACTG1, AKT1, AKT2, BAD, BCL2, BRAF, CCND1, CRK, CTNNB1, EGFR, ELK1, ERBB2, GSK3B, IGF1R, ITGB3, KDR, MAP2K1, MAKP9, MET, PAK1, PDGFRB, PDPK1, PPP1CA, PTEN, PTK2, RAF1, RASGRF1, SHC1, SRC, VASP

Insulin
ACACA, AKT1, AKT2, BAD, BRAF, CALM1, CBL, CRK, EIF4E, EIF4EBP1, ELK1, FOXO1, GSK3B, IKBKB, IRS1, MAK2K1, MAPK9, MTOR, PDPK1, PPP1CA, PRKAA1, RAF1, RPS6KB1, SHC1, SREBF1, TSC2 ,

JAK-STAT
AKT1, AKT2, BCL2, CCND1, CDKN1A, EGFR, IFNGR1, IL10RA, IL4R, JAK1, JAK2, MTOR, MYC, PDGFRB, PTPN11, RAF1, STAT1, STAT2, STAT3, STAT4, STAT5A, STAT5B, STAT6

MAPK
AKT1, AKT2, BRAF, CHUK, CRK, EGFR, ELK1, ERBB2, FOS, HSPB1, IGR1R, IKBKG, KDR, MAP2K1, MAP2K4, MAP3K5, MAP3K7, MAPK14, MAPK9, MAPT, MET, MYC, NFKB1, NTRK2, PAK1, PDFGRB, RAF1, RASGRF1, RELA, RELB, RPS6KA1, RPS6KA2, RPS6KA3, RPS6KA5, SRF, TP53, CASP3

mTOR
AKT1, AKT1S1, AKT2, BRAF, CHUK, EIF4E, EIF4EBP1, GSK3B, IFG1R, IKBKB, IRS1, MAP2K1, MTOR, PDPK1, PRKAA1, PTEN, RAF1, RPS6KA1, RPS6KA2, RPS6KA3, RPS6KB1, STK11, TSC2, WNT1

NF-kappa B
BCL2, BLNK, CHUK, IKBKB, IKBKG, LCK, LYN, MAP3K7, NFKB1, NFKB1A, PLCG1, RELA, RELB, SYK, ZAP70

p53
BAX, BCL2, BID, CASP8, CASP9, CCND1, CCNE1, CDK2, CDKNIA, CHEK1, CHEK2, MDM2, PTEN, TP53, TP73, TSC2, CASP3, CASP8

Ras
ABL1, AKT1, AKT2, BAD, CALM1, CHUK, EGFR, ELK1, GAB1, GAB2, GRIN1, IGF1R, IKBKB, IKBKG, KDR, MAP2K1, MAPK9, MET, NFKB1, PAK1, PDFGRB, PLCG1, PTPN11, RAF1, RASGRF1, RELA, SHC1, ZAP70

TGF-beta
MYC, PPP2CA, RPS6KB1, SMAD1, SMAD2, SMAD3, TGFBR1, TGFBR2

VEGF
AKT1, AKT2, BAD, CASP9, HSPB1, MAP2K1, MAPK14, NOS3, PLCG1, PTK2, RAF1, SRC, VEGFR2

The ELISA based Cell Signaling Phospho Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Antibody Array - How It Works

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Chalise U, Becirovic-Agic, MMP-12 polarizes the neutrophil signalome towards an apoptotic signature, J. Proteomics. 2022. https://doi.org/10.1016/j.jprot.2022.104636

Choe YJ, Min JY, Heterotypic cell-in-cell structures between cancer and NK cells is associated with
enhanced anti-cancer drug resistance, iScience. 2002 Aug 27, 105017. https://doi.org/10.1016/j.isci.2022.105017

Sun X, Chen M, Knockdown of KIF15 promotes cell apoptosis by activating crosstalk of multiple pathways in ovarian cancer: bioinformatic and experimental analysis, Int J Clin Exp Pathol. 2021 Feb 1;14(2):267-291

Wang H, Ge L, 3,4,5-Tri-O-caffeoylquinic acid methyl ester isolated from Lonicera japonica Thunb. Flower buds facilitates hepatitis B virus replication in HepG2.2.15 cells, Food Chem Toxcol2020 March 7. doi: 10.1016/j.fct.2020.111250

Ye Y, Wang H, Polygalasaponin F treats mice with pneumonia induced by influenza virus, Inflammopharmacology. 2019 Aug 24. doi: 10.1007/s10787-019-00633-1

ADDITIONAL SERVICES

If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.

Cost: Free

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $300 per slide

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,730 per sample

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