mTOR Phospho Antibody Array

mTOR Phospho Antibody Array

mTOR Antibody Array features 138 highly specific antibodies related to the mTOR pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PMT138

$500.00

mTOR Phospho Antibody Array

The mechanistic target of rapamycin (mTOR) is a serine-threonine protein kinase that regulates cell growth, cell proliferation, protein synthesis, and autophagy. Abnormal mTOR signaling has been associated with neurological disorders, cancer, and diabetes. mTOR forms two distinct complexes: mTOR Complex 1 (mTORC1) and mTOR Complex 2 (mTORC2). mTOR binds with the regulatory protein raptor and other binding proteins to form mTORC1 while it binds with regulatory protein rictor and other proteins to form mTORC2. mTORC1 phosphorylates p70S6K and eIF4E to promote mRNA translation and protein synthesis. Growth factors such as insulin trigger the AKT pathway, which signals downstream to mTORC1 to promote cell growth and inhibit autophagy. Oppositely, nutrient deprivation and low energy levels activate the AMPK pathway, which inhibits mTORC1, thus restricting cell growth and promoting autophagy. mTORC2 can affect metabolism and cell survival through phosphorylation of AKT.  mTORC2 regulates the cytoskeleton through PKC alpha activation and also influences ion transport.

mTOR Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine, serine, and threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening
  • Suitable samples include: cell lysates; frozen or FFPE tissue lysates
  • Glass based array with high specificity and low background
  • Antibodies covalently attached to 3D polymer coated glass surface
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 138 site-specific and phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 97%; Rat: 83%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months

 

 Selected Publications

Arsenault, R, Napper, S, Salmonella enterica Typhimurium infection causes metabolic changes in chicken muscle involving AMPK, fatty acid and insulin/mTOR signaling, Veterinary Research 2013, 44:35

Wang H, Brown J, et al.: Convergence of the Mammalian Target of Rapamycin Complex 1- and Glycogen Synthase Kinase 3-b–Signaling Pathways Regulates the Innate Inflammatory Response, Journal of Immunology 2011, 186:5217-5226

Ordering Information 
Catalog No Description Size Price
PMT138 mTOR Phospho Antibody Array 2 slides/pk $500
Additional Reagent Needed:

The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated streptavidin.Antibody Array- How It Works

 Questions? Visit our Frequently Asked Questions page.

 

4E-BP1 (Ser65), 4E-BP1 (Thr36), 4E-BP1 (Thr45), 4E-BP1 (Thr70), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT1S1 (Thr246), AKT2 (Ser474), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AMPK1/AMPK2 (Ser485/491), AMPK1 (Thr174), AMPKbeta1 (Ser182), BAD (Ser112), BAD (Ser134), BAD (Ser136), BAD (Ser155), BAD (Ser91/128), eIF2A (Ser51), eIF4B (Ser422), eIF4E (Ser209), eIF4G (Ser1108), ERK1/2, ERK1 (Thr202), ERK1 (Tyr204), ERK3 (Ser18), ERK8 (Thr175/Tyr177), GSK3a (Ser21), GSK3a-b (Tyr216/270), GSK3b (Ser9), IR (Tyr1355), IR (Tyr1361), Mnk1 (Thr385), mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), P70S6K beta (Ser423), P70S6K (Ser371), P70S6K (Ser411), P70S6K (Ser418), P70S6K (Ser424), P70S6K (Thr229), P70S6K (Thr389), P70S6K (Thr421), P90RSK (Ser380), P90RSK (Thr359/Ser363), P90RSK (Thr573), PDK1 (Ser241), PFKFB2 (Ser483), PI3K-p85-a (Tyr607), PI3K-p85-a/g (Tyr467/Tyr199), PIP5K (Ser307), PKC alpha (Tyr657), PKC alpha/beta II (Thr638), PP2A-alpha (Tyr307), PPAR-b (Thr1457), PPAR-g (Ser112), PTEN (Ser370), PTEN (Ser380), PTEN (Ser380/Thr382/Thr383), Rho/Rac GEF2 (Ser885), RSK1/2/3/4 (Ser221/227/218/232), Tuberin (Ser939), Tuberin (Thr1462)

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

Arsenault, R, Napper, S, Salmonella enterica Typhimurium infection causes metabolic changes in chicken muscle involving AMPK, fatty acid and insulin/mTOR signaling, Veterinary Research 2013, 44:35

Wang H, Brown J, et al.: Convergence of the Mammalian Target of Rapamycin Complex 1- and Glycogen Synthase Kinase 3-b–Signaling Pathways Regulates the Innate Inflammatory Response, Journal of Immunology 2011, 186:5217-5226

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,300 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $200 per slide

 

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