T-Cell Receptor Phospho Antibody Array

T-Cell Receptor Phospho Antibody Array

T-Cell Receptor Phospho Antibody Array features 213 highly specific antibodies related to T-cell receptors. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog No: PTC188

$730.00

T-Cell Receptor Phospho Antibody Array

The newly updated T-Cell Receptor Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein expression profiling. It is suitable for comparing protein expressions between non-treated and treated samples and identifying candidate biomarkers related to TCR signaling. This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine, serine, and threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening with 213 antibodies
  • Antibodies covalently immobilized on 3D polymer coated glass slide
  • Fluorescent detection
Specifications
Product Size:2 array slides per package for analyzing two samples (control vs. treated)
Featured Antibodies:213 site-specific and phospho-specific antibodies; 6 replicates per antibody
Reactivity:Human: 99% | Mouse: 82% | Rat: 55%
Suitable Sample Type:Cell lysate | Tissue lysate
Detection Method:Fluorescence | Compatible Scanners
Internal Controls:beta-actin | GAPDH | Negative controls
Slide dimensions: 76 x 25 x 1 mm
Storage Condition:4°C for 6 months
PRODUCT DETAILS

AKT(Ser473), AKT(Thr308), AKT(Tyr326), Calmodulin(Thr79/Ser81), CD127, CD134 (OX40), CD137, CD152, CD154, CD197 (CCR7), CD2, CD25, CD27, CD272, CD278 (ICOS), CD279, CD28, CD28(Tyr218), CD3, CD39, CD3Z(Tyr142), CD4, CD4(Ser433), CD45(Ser1007), CD45RA, CD45RO, CD5, CD62L, CD69, CD7, CD73, CD8, c-Jun(Ser243), c-Jun(Ser63), c-Jun(Ser73), c-Jun(Thr239), c-Jun(Thr91), c-Jun(Thr93), c-Jun(Tyr170), ERK1(Thr202), ERK1(Tyr204), ERK1/2, ERK3(Ser189), ERK8(Thr175/Tyr177), Fos(Ser362), Fos(Thr232), FosB(Ser27), GAD1/GAD2, GAD67/GAD1, GADD153, GRB2(Ser159), IkB-alpha(Ser32/36), IkB-alpha(Tyr305), IkB-alpha(Tyr42), IkB-beta(Thr19), IkB-epsilon(Ser22), IKK alpha(Thr23), IKK alpha/beta(Ser180/181), IKK beta(Tyr188), IKK beta(Tyr199), IKK gamma(Ser31), IKK gamma(Ser85), IP3KA, IP3KC, JNK1/2/3(Thr183/Tyr185), LAT(Tyr171), LAT(Tyr191), LAT3, LCK(Ser59), LCK(Tyr192), LCK(Tyr393), LCK(Tyr504), MEK1(Ser217), MEK1(Ser221), MEK1(Ser298), MEK1(Thr286), MEK1(Thr291), MEK2(Thr394), MEKKK1, MEKKK4, MKK3(Ser189), MKK3/MAP2K3(Thr222), MKK6(Ser207), MKK7/MAP2K7(Ser271), MKK7/MAP2K7(Thr275), NFAT3, NFAT4(Ser165), NFkB-p100/p52(Ser865), NFkB-p100/p52(Ser869), NFkB-p105(Ser927), NFkB-p105/p50(Ser337), NFkB-p105/p50(Ser932), NFkB-p65(Ser536), NFkB-p65(Thr254), p38 MAPK(Thr180), p38 MAPK(Tyr182), p38 MAPK(Tyr322), Perforin, PI3K-p85-a(Tyr607), PI3K-p85-a/g(Tyr467/199), PKC pan activation sites, PKC theta(Ser676), PKC theta(Thr538), Rac1/cdc42(Ser71), Raf1(Ser259), Raf1(Ser338), Raf1(Ser621), Raf1(Tyr341), Ras-GRF1(Ser916), Rel(Ser503), RelB(Ser552), SLP-76(Tyr128), VAV(Tyr174), VAV2(Tyr142), WASP(Tyr290), ZAP-70(Tyr292), ZAP-70(Tyr315), Zap-70(Tyr319), Zap-70(Tyr493)

The ELISA based T-Cell Receptor Phospho Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Antibody Array - How It Works

Antibody Array User’s Guide

Antibody List

Array Map

GAL File  (To download, right click on the file name, then choose “Save target as”)

Material Safety Data Sheet (MSDS)

Frequently Asked Questions

ADDITIONAL SERVICES

If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.

Cost: Free

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $250 per slide

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,520 per sample

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