Cell Cycle Antibody Array

Cell Cycle Antibody Array

Cell Cycle Antibody Array features 60 highly specific and well-characterized antibodies in the cell cycle pathway. It is designed for protein profiling in human cell lysates, tissue lysates, serum, or culture media. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: ACC058

$320.00

Cell Cycle Antibody Array

Cell Cycle Antibody Array is a high-throughput ELISA based antibody array, designed for qualitative/semi-quantitative protein expression profiling, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.

Key Features
  • Qualitative/semi-quantitative protein expression profiling
  • Suitable samples: cell lysates; frozen or FFPE tissue lysates; serum; culture media
  • Glass based array with high specificity and low background
  • Antibodies are covalently attached to 3D polymer coated glass slides
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 60 antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months

 

Selected Publications

Sobol A, Galluzzo P, Depletion of Amyloid Precursor Protein (APP) Causes G0 Arrest in Non-Small Cell Lung Cancer (NSCLC) CellsJournal of Cellular Physiology, 2015 230(6): 1332–1341

Tian X, Jin X, The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway, Mol Cancer 2017, 16:74

Ordering Information
Catalog No Description Size Price
ACC058 Cell Cycle Antibody Array 2 slides/pk $320
Additional Reagent Needed:

The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

Cell Cycle Antibody Array

 Questions? Visit our Frequently Asked Questions page.

14.3.3 Pan, APC11, APC2, ATM, c-Abl, CDC14A Phosphatase, CDC6, CDC25C, CDC34, CDC37, CDC47, Cdh1, Cdk1/p34cdc2, Cdk2 , Cdk3 , Cdk4 , Cdk5 , Cdk7, Cdk8, Chk1, Cullin-1, Cullin-2, Cullin-3, Cyclin B1, Cyclin C, Cyclin D1, Cyclin D2, Cyclin E, Cyclin E2, E2F-1, E2F-2, E2F-3, Glycogen Synthase Kinase 3b (GSK3b), Ki67, Mitochondria, NuMA, p130, p130cas, p14ARF, p15INK4b, p16INK4a, p18INK4c, p19ARF, p19Skp1, p21WAF1, p27Kip1, p35nck5a, p53, p57Kip2, p73, p73a, p73a/b, PCNA, RAD 51, Retinoblastoma, Retinoblastoma (Phospho-specific Ser608), ROC, Topo II beta, Tubulin-a, Tubulin-b

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

Chen P, Huang H, Bone marrow stromal cells protect acute myeloid leukemia cells from anti-CD44 therapy partly through regulating PI3K/Akt–p27Kip1 axis. Molecular Carcinogenesis 2014, doi: 10.1002/mc.22239

Sobol A, Galluzzo P, Depletion of Amyloid Precursor Protein (APP) Causes G0 Arrest in Non-Small Cell Lung Cancer (NSCLC) CellsJournal of Cellular Physiology 2015, 230(6): 1332–1341

Tian X, Jin X, The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway, Mol Cancer 2017, 16:74

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,100 per sample

Array Scanning & Image Analysis Service

Don’t have access to a compatible microarray scanner? No problem! Once the assays are completed, send the finished array slides to our lab for scanning (free of charge) and analysis.

 

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