Tyrosine Phosphorylation ProArray

Tyrosine Phosphorylation ProArray

Tyrosine Phosphorylation ProArray features 228 site-specific phospho-tyrosine antibodies.  This antibody array is specifically designed for site-specific tyrosine phosphorylation profiling in cell and tissue samples. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PST228

$650.00

Tyrosine Phosphorylation ProArray

Tyrosine Phosphorylation ProArray is a high-throughput ELISA based antibody array for qualitative tyrosine phosphorylation profiling. It is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features 228 site-specific phospho-tyrosine antibodies, allowing researchers to study tyrosine phosphorylation at specific sites.

  • Site-specific tyrosine phosphorylation profiling and screening
  • Suitable samples: cell lysates; frozen or FFPE tissue lysates
  • Glass based array with high specificity and low background
  • Antibodies covalently attached to 3D polymer coated glass surface
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 228 tyrosine phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 95%; Rat: 62%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months

 

Selected Publications

Ding Y, Wu M, Seed-targeting anti-miR-21 inhibiting malignant progression of retinoblastoma and analysis of their phosphorylation signaling pathways, Experimental Eye Research, May 2014, 122: 1-8

Makishima H, Sugimoto Y, CBL mutation-related patterns of phosphorylation and sensitivity to tyrosine kinase inhibitors, Leukemia 2012, Jul;26(7):1547-54

Xu N, Yuan H, Activation of RAW264.7 mouse macrophage cells in vitro through treatment with recombinant ricin toxin-binding subunit B: Involvement of protein tyrosine, NF-κB and JAK-STAT kinase signaling pathways. International Journal of Molecular Medicine 2013 Sep;32(3):729-35

Ordering Information
Catalog No Description Size Price
PST228 Tyrosine Phosphorylation ProArray 2 slides/pk $650
Additional Reagent Needed:

The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

Antibody Array - How It Works

 Questions? Visit our Frequently Asked Questions page.

Abl1 (pTyr204), Abl1 (pTyr412), ACK1 (pTyr284), ACTIN Pan (a/b/g) (pTyr55/53), AKT (pTyr326), AKT1 (pTyr474), ALK (pTyr1507), ALK (pTyr1604), A-RAF (pTyr301/302), AurB (pTyr12), AXL (pTyr691), Bcr (pTyr177), BCR (pTyr360), BLNK (pTyr84), BLNK (pTyr96), Breast tumor kinase (pTyr447), BTK (pTyr222), BTK (pTyr550), c-Abl (pTyr245), c-Abl (pTyr412), Caspase 9 (pTyr153), Catalase (pTyr385), Catenin beta (pCTNNB) (pTyr489), Catenin beta (pTyr654), Catenin delta-1 (pTyr228), Caveolin-1 (pTyr14), CBL (pTyr700), CBL (pTyr774), CD19 (pTyr531), CD22/BL-CAM (pTyr807), CD227/mucin 1 (pTyr1243), CD28 (pTyr218), CD3Z (pTyr142), CD5 (pTyr453), CDC2 (pTyr15), CDK5 (pTyr15), c-Jun (pTyr170), CK1-A/A2 (pTyr294), c-Kit (pTyr721), claudin 3 (pTyr219), claudin 6 (pTyr219), claudin 7 (pTyr210), c-met (pTyr1003), Cortactin (pTyr421), Cortactin (pTyr466), CrkII (pTyr221), CrkL (pTyr207), CSFR (pTyr561), Dab1 (pTyr220), Dab1 (pTyr232), DAPP1 (pTyr139), DDX5/DEAD-box protein 5 (pTyr593), Dok1 (pTyr362), Dok1 (pTyr398), Dok2 (pTyr299), EGFR (pTyr1016), EGFR (pTyr1069), EGFR (pTyr1092), EGFR (pTyr1110), EGFR (pTyr1172), EGFR (pTyr1197), EGFR (pTyr869), EPB41 (pTyr418/660), EPHA2/3/4 (pTyr588/596), EPHB1/2 (pTyr594/604), Ephrin B (pTyr330), Ephrin B1/B2/B3 (pTyr324), Ephrin-B1 (pTyr317), Epo-R (pTyr368), ERK8 (pThr175/177), Etk (pTyr40), ETK (pTyr566), Ezrin (pTyr353), ezrin (pTyr478), FAK (pTyr397), FAK (pTyr407), FAK (pTyr576), FAK (pTyr861), FAK (pTyr925), FER (pTyr402), FGFR1 (pTyr154), FGFR1 (pTyr654), FGFR1 (pTyr766), FLT3 (pTyr842), FLT3 (pTyr969), FRS2 (pTyr436), Fyn (pTyr530), Gab1 (pTyr627), Gab1 (pTyr659), Gab2 (pTyr643), GRB10 (pTyr67), GRF-1 (pTyr1105), GSK3a-b (pTyr216/279), HCK (pTyr410), HER2 (pTyr1221/1222), HER2 (pTyr1248), HER2 (pTyr877), HER3/ErbB3 (pTyr1222), HER3/ErbB3 (pTyr1289), HER4/ErbB4 (pTyr1284), HRS (pTyr334), ICAM-1 (pTyr512), ICK (pTyr159), IGF-1R (pTyr1161), IGF-1R (pTyr1165/1166), IkB-alpha (pTyr305), IkB-alpha (pTyr42), IKK-beta (pTyr188), IKK-beta (pTyr199), IL-10R-A (pTyr496), IL-13R/CD213a1 (pTyr405), IL3R (pTyr593), IL-4R/CD124 (pTyr497), IL7R/CD127 (pTyr449), Integrin b3 (pTyr773), Integrin b3 (pTyr785), IFN-gamma receptor alpha chain precursor (pTyr457), IR (pTyr1355), IR (pTyr1361), ITGB4 (pTyr1510), JAK1 (pTyr1022), JAK2 (pTyr1007), JAK2 (pTyr221), JNK1/2/3 (pThr183/Tyr185), KIT (pTyr703), KIT (pTyr936), Kv1.3/KCNA3 (pTyr135), Kv2.1/Kcnb1 (pTyr128), LAT (pTyr171), LAT (pTyr191), LCK (pTyr192), LCK (pTyr393), LCK (pTyr504), LYN (pTyr507), M-CSF Receptor (pTyr809), MER/SKY (pTyr749/681), Met (pTyr1234), Met (pTyr1349), Met (pTyr1356), NMDA NR2A/B (pTyr1246/1252), NMDAR2B (pTyr1472), p130Cas (pTyr165), p130Cas (pTyr410), p38 MAPK (pTyr182), p38 MAPK (pTyr322), p44/42 MAPKinase (pTyr204), p73 (pTyr99), Paxillin (pTyr118), Paxillin (pTyr31), PDGF-R alpha (pTyr849), PDGF-R beta (pTyr1021), PDGF-R beta (pTyr740), PDGF-R beta (pTyr751), PECAM-1 (pTyr713), PI3-kinase p85-alpha (pTyr607), PI3-kinase p85-subunit a/g (pTyr467/199), Pim-1 (pTyr309), PKC alpha (pTyr657), PKC delta (pTyr52), PKC delta (pTyr313), PKC delta/PKCD (pTyr64), PKD1/PKC mu (pTyr463), PLCG1 (pTyr1253), PLCG1 (pTyr771), PLCG1 (pTyr783), PLCG2 (pTyr1217), PLCG2 (pTyr753), PLD2 (pTyr169), PP2A-a (pTyr307), PTPRA (pTyr798), Pyk2 (pTyr402), Pyk2 (pTyr579), Pyk2 (pTyr580), Pyk2 (pTyr881), RAD51 (pTyr315), RAD52 (pTyr104), Raf1 (pTyr341), RapGEF1 (pTyr504), Ret (pTyr905), RGS16 (pTyr168), SAPK/JNK (pTyr185), Shc (pTyr349), Shc (pTyr427), SHP-1 (pTyr536), SHP-2 (pTyr542), SHP-2 (pTyr580), SLP-76 (pTyr128), Src (pTyr216), Src (pTyr418), Src (pTyr529), STAM2 (pTyr192), STAT1 (pTyr701), STAT2 (pTyr690), STAT3 (pTyr705), STAT4 (pTyr693), STAT5A (pTyr694), STAT6 (pTyr641), SYK (pTyr323), SYK (pTyr348), SYK (pTyr525), Synuclein alpha (pTyr125), Synuclein alpha (pTyr133), Synuclein alpha (pTyr136), TFII-I (pTyr248), TIE2 (pTyr1108), Trk A (pTyr680/681), Trk A (pTyr701), Trk A (pTyr791), Trk B (pTyr515), Trk B (pTyr705), TYK2 (pTyr1054), Vav1 (pTyr174), Vav2 (pTyr142), VE-Cadherin (pTyr731), VEGFR1 (pTyr1333), VEGFR2 (pTyr1054), VEGFR2 (pTyr1059), VEGFR2 (pTyr1175), VEGFR2 (pTyr1214), VEGFR2 (pTyr951), Vinculin (pTyr821), WASP (pTyr290), WAVE1 (pTyr125), WWOX (pTyr33), ZAP-70 (pTyr292), ZAP-70 (pTyr315), ZAP-70 (pTyr319), ZAP-70 (pTyr493)

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

Ding Y, Wu M, Seed-targeting anti-miR-21 inhibiting malignant progression of retinoblastoma and analysis of their phosphorylation signaling pathways, Experimental Eye Research, May 2014, 122: 1-8

Makishima H, Sugimoto Y, CBL mutation-related patterns of phosphorylation and sensitivity to tyrosine kinase inhibitors, Leukemia 2012, Jul;26(7):1547-54

Xu N, Yuan H, Activation of RAW264.7 mouse macrophage cells in vitro through treatment with recombinant ricin toxin-binding subunit B: Involvement of protein tyrosine, NF-κB and JAK-STAT kinase signaling pathways, International Journal of Molecular Medicine 2013 Sep;32(3):729-35

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,350 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $250 per slide

 

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