MAPK Phospho Antibody Array

MAPK Phospho Antibody Array

MAPK Pathway Phospho Antibody Array features 185 highly specific antibodies related to the MAPK pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PMK185

$580.00

MAPK Pathway Phospho Antibody Array

MAPK Pathway Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine phosphorylation and serine/threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening
  • Suitable samples include: cell lysates; frozen or FFPE tissue lysates
  • Glass based array with high specificity and low background
  • Antibodies covalently attached to 3D polymer coated glass surface
  • Sensitive fluorescent detection
User’s Guide  

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 185 site-specific and phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 85%; Rat: 71%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months
Recent Publications

Feng Y, He F, The Role of GOLPH3L in the Prognosis and NACT response in Cervical CancerJournal of Cancer 2017, 8(3):443-454

Genebai N, Ahmad S, Genetic inhibition of JNK3 ameliorates spinal muscular atrophyHuman Molecular Genetics, First published online: September 30, 2015

Ordering Information 
Catalog No Description Size Price
PMK185 MAPK Pathway Phospho Antibody Array 2 slides/pk $580
Additional Reagent Needed:

The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated streptavidin.Antibody Array- How It Works

 Questions? Visit our Frequently Asked Questions page.

 

4E-BP1 (Thr36), 4E-BP1 (Thr45), ASK1 (Ser83), ASK1 (Ser966), ATF2 (Ser112/94), ATF2 (Ser62/44), ATF2 (Thr69/51), ATF2 (Thr71/53), ATF2 (Thr73/55), ATF4 (Ser245), c-Jun (Ser243), c-Jun (Ser63), c-Jun (Ser73), c-Jun (Thr239), c-Jun (Thr91), c-Jun (Thr93), c-Jun (Tyr170), c-Kit (Tyr721), CREB (Ser129), CREB (Ser133), eIF2 alpha (Ser51), elF4E (Ser209), Elk1 (Ser383), Elk1 (Ser389), Elk1 (Thr417), Estrogen Receptor-a (Ser104), Estrogen Receptor-a (Ser106), Estrogen Receptor-a (Ser118), Estrogen Receptor-a (Ser167), FAK (Tyr861), FAK (Tyr925), Histone H2A.X (Ser139), Histone H3.1 (Ser10), HSF1 (Ser303), HSP27 (Ser15), HSP27 (Ser78), HSP27 (Ser82), IRS-1 (Ser307), IRS-1 (Ser312), IRS-1 (Ser636), IRS-1 (Ser639), JunB (Ser259), JunB (Ser79), JunD (Ser255), MEF2A (Thr312), MEF2A (Thr319), MEK1 (Ser217), MEK1 (Ser221), MEK1 (Thr291), MEK2 (Thr394), Met (Tyr1234), Met (Tyr1349), MKK3 (Ser189), Myc (Ser373), Myc (Thr358), Myc (Thr58), P38 MAPK (Thr180), P38 MAPK (Tyr182), p44/42 MAPK (Thr202), p44/42 MAPK (Tyr204), p53 (Ser15), p53 (Ser315), p53 (Ser33), p53 (Ser37), p53 (Ser46), p53 (Ser6), p53 (Ser9), p53 (Thr18), PKC delta (Ser645), PKC theta (Ser676), Pyk2 (Tyr402), Raf1 (Ser259), Raf1 (Ser338), SAPK/JNK (Thr183), SAPK/JNK (Tyr185), SEK1/MKK4 (Ser80), SEK1/MKK4 (Thr261), Src (Tyr418), Src (Tyr529), Stathmin1 (Ser15), Stathmin1 (Ser24), Stathmin1 (Ser37), Tau (Ser214), Tau (Ser235), Tau (Ser262), Tau (Ser356), Tau (Ser396), Tau (Ser404), Tau (Ser422), Tau (Thr181), Tau (Thr205), Tau (Thr212), Tau (Thr231)

 

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

Genebai N, Ahmad S, Genetic inhibition of JNK3 ameliorates spinal muscular atrophyHuman Molecular Genetics, First published online: September 30, 2015

Feng Y, He F, The Role of GOLPH3L in the Prognosis and NACT response in Cervical CancerJournal of Cancer 2017, 8(3):443-454

Jin L, Li D, p90 RSK2 Mediates Antianoikis Signals by both Transcription-Dependent and -Independent Mechanisms, Molecular and Cellular Biology 2013, 33(13):2574-2585

Raharijaona M, Le Pennec S, et al. PGC-1-Related Coactivator Modulates Mitochondrial-Nuclear Crosstalk through Endogenous Nitric Oxide in a Cellular Model of Oncocytic Thyroid Tumours, PLoS ONE 2009; 4(11):e7964.

Rao W, Li H, OVA66 Increases Cell Growth, Invasion, and Survival via Regulation of IGF-1R-MAPK Signaling in Human Cancer CellsCarcinogenesis (2014) 35 (7):1573-1581

Yu Y, Richardson DR, Cellular iron depletion stimulates the JNK and p38 MAPK signaling transduction pathways, dissociation of ASK1-thioredoxin, and activation of ASK1, Journal of Biological Chemistry 2011, 286(17):15413-27

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,300 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $200 per slide

 

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