MAPK Signaling Antibody Array

MAPK Signaling Antibody Array

MAPK Antibody Array is designed for expression profiling of key proteins in MAP Kinase signaling cascades. It is suitable for total protein profiling in human cell lysates, tissue lysates, serum, or culture media. Each package contains two identical array slides for analyzing two samples, such as an untreated sample and a treatment sample.

Catalog #: SMK060

$330.00

MAP Kinase (MAPK) Signaling Antibody Array 

The mitogen-activated protein kinase (MAPK) Antibody Array is an ELISA based antibody array for studying MAP kinase signaling cascades. It is designed for qualitative/semi-quantitative protein expression profiling, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.

Key Features
  • Qualitative/semi-quantitative protein expression profiling
  • Suitable samples: cell lysates; frozen or FFPE tissue lysates; serum; culture media
  • Glass based array with high specificity and low background
  • Antibodies are covalently attached to 3D polymer coated glass slides
  • Each antibody is printed with six replicates
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 63 antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months

 

Ordering Information 
Catalog No Description Size Price
SMK060 MAPK Signaling Antibody Array 2 slides/pk $330
Additional Reagent Needed:

The ELISA based MAP Kinase (MAPK) antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

MAP Kinase (MAPK) Antibody Array

 Questions? Visit our Frequently Asked Questions page.

 

AKT1, AKT2, Akt3, A-RAF, ASK1 (MAP3K5), ASK2 (MAP3K6), ASK3 (MAP3K15), BRAF, c-RAF, CREB, Elk1, ERK1 (MAPK3, p44-MAPK), ERK2 (MAPK1, p42-MAPK), ERK3 (MAPK6), ERK4 (MAPK4, p63MAPK), ERK7 (MAPK15), GSK3 alpha, GSK3 beta, HGK (MAP4K4), HSP27, JNK1 (MAPK8), JNK2 (MAPK9), JNK3 (MAPK10), KHS1 (MAP4K5), KHS2 (MAP4K3), LAMTOR3 (MAP2K1IP1), MAP2K4, MEK1 (MAP2K1), MEK2 (MAP2K2), MEK3 (MAP2K3), MEK5 (MAP2K5), MEK6 (MAP2K6), MEKK1 (MAP3K1), MEKK3 (MAP3K3), MEKK4 (MAP3K4), MINK (MAP4K6), MK2 (MAPKAPK2), MK3 (MAPKAPK3), MLK (MAP3K13), MLK1 (MAP3K9), MLK2 (MAP3K10), MLK3 (MAP3K11), MLK4 (MAP3KL4), MSK1 (RPS6KA5), mTOR, NF-kB p65, p38A (MAPK14), p38B (MAPK11), p38D (MAPK13), p38G (MAPK12), p44/42 MAPK, p53, p70S6K (RPS6KB1), p70S6K2 (RPS6KB2), PRAK (MAPKAPK5), RSK1 (RPS6KA1), RSK2 (RPS6KA3), RSK3 (RPS6KA2), RSK4 (RPS6KA6), RSKL1 (RPS6KC1), STAT1, TAB1 (MAP3K7IP1), TAK1 (MAP3K7)

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

Selected Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,100 per sample

Array Scanning & Image Analysis Service

Don’t have access to a compatible microarray scanner? No problem! Once the assays are completed, send the finished array slides to our lab for scanning (free of charge) and analysis.

 

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