MAPK Signaling Antibody Array
MAPK Antibody Array is designed for expression profiling of key proteins in MAP Kinase signaling cascades. It is suitable for total protein profiling in human cell lysates, tissue lysates, serum, or culture media. Each package contains two identical array slides for analyzing two samples, such as an untreated sample and a treatment sample.
MAP Kinase (MAPK) Signaling Antibody Array
The mitogen-activated protein kinase (MAPK) Antibody Array is an ELISA based antibody array for studying MAP kinase signaling cascades. It is designed for qualitative/semi-quantitative protein expression profiling, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.
- Qualitative/semi-quantitative protein expression profiling
- Suitable samples: cell lysates; frozen or FFPE tissue lysates; serum; culture media
- Glass based array with high specificity and low background
- Antibodies are covalently attached to 3D polymer coated glass slides
- Each antibody is printed with six replicates
- Sensitive fluorescent detection
|Number of Antibodies:||63 antibodies|
|Number of Replicates:||6 replicates per antibody|
Detailed Reactivity List
|Internal Controls:||Positive controls: beta-actin; GAPDH
|Slide dimensions:||76 x 25 x 1 mm|
|Spot diameter:||260 – 280 um|
|Size:||2 array slides per package for analyzing two samples (control vs. treated)|
|Storage Condition:||4°C for 6 months|
|SMK060||MAPK Signaling Antibody Array||2 slides/pk||$330|
Additional Reagent Needed:
The ELISA based MAP Kinase (MAPK) antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated streptavidin.
Questions? Visit our Frequently Asked Questions page.
AKT1, AKT2, Akt3, A-RAF, ASK1 (MAP3K5), ASK2 (MAP3K6), ASK3 (MAP3K15), BRAF, c-RAF, CREB, Elk1, ERK1 (MAPK3, p44-MAPK), ERK2 (MAPK1, p42-MAPK), ERK3 (MAPK6), ERK4 (MAPK4, p63MAPK), ERK7 (MAPK15), GSK3 alpha, GSK3 beta, HGK (MAP4K4), HSP27, JNK1 (MAPK8), JNK2 (MAPK9), JNK3 (MAPK10), KHS1 (MAP4K5), KHS2 (MAP4K3), LAMTOR3 (MAP2K1IP1), MAP2K4, MEK1 (MAP2K1), MEK2 (MAP2K2), MEK3 (MAP2K3), MEK5 (MAP2K5), MEK6 (MAP2K6), MEKK1 (MAP3K1), MEKK3 (MAP3K3), MEKK4 (MAP3K4), MINK (MAP4K6), MK2 (MAPKAPK2), MK3 (MAPKAPK3), MLK (MAP3K13), MLK1 (MAP3K9), MLK2 (MAP3K10), MLK3 (MAP3K11), MLK4 (MAP3KL4), MSK1 (RPS6KA5), mTOR, NF-kB p65, p38A (MAPK14), p38B (MAPK11), p38D (MAPK13), p38G (MAPK12), p44/42 MAPK, p53, p70S6K (RPS6KB1), p70S6K2 (RPS6KB2), PRAK (MAPKAPK5), RSK1 (RPS6KA1), RSK2 (RPS6KA3), RSK3 (RPS6KA2), RSK4 (RPS6KA6), RSKL1 (RPS6KC1), STAT1, TAB1 (MAP3K7IP1), TAK1 (MAP3K7)
GAL File (To download, right click on the file name, then choose “Save target as”)
Complete Antibody Array Assay Service
Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.
Cost: $1,100 per sample
Array Scanning & Image Analysis Service
Don’t have access to a compatible microarray scanner? No problem! Once the assays are completed, send the finished array slides to our lab for scanning (free of charge) and analysis.