MAPK Signaling Antibody Array

MAPK Signaling Antibody Array

MAPK Antibody Array is designed for expression profiling of key proteins in MAP Kinase signaling cascades. It is suitable for total protein profiling in human cell lysates, tissue lysates, serum, or culture media. Each package contains two identical array slides for analyzing two samples, such as an untreated sample and a treatment sample.

Catalog No: SMK060

$380.00

MAP Kinase (MAPK) Signaling Antibody Array

The mitogen-activated protein kinase (MAPK) Antibody Array is an ELISA based antibody array for studying MAP kinase signaling cascades. It is designed for qualitative/semi-quantitative protein expression profiling, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.

Key Features
  • Qualitative/semi-quantitative protein expression profiling with 60 antibodies
  • Antibodies covalently immobilized on 3D polymer coated glass slide
  • Fluorescent detection
Specifications
Product Size:2 array slides per package for two samples (untreated vs. treated)
Featured Antibodies:63 antibodies; 6 replicates per antibody
Reactivity:Human
Suitable Sample Type:Cell lysate | Tissue lysate | Serum | Culture Media
Detection Method:Fluorescence | Compatible Scanners
Internal Controls:Positive controls: beta-actin | GAPDH | Negative controls
Slide dimensions: 76 x 25 x 1 mm
Storage Condition:4°C for 6 months

 

PRODUCT DETAILS

AKT1, AKT2, Akt3, A-RAF, ASK1 (MAP3K5), ASK2 (MAP3K6), ASK3 (MAP3K15), BRAF, c-RAF, CREB, Elk1, “ERK1 (MAPK3, p44-MAPK)”, “ERK2 (MAPK1, p42-MAPK)”, ERK3 (MAPK6), “ERK4 (MAPK4, p63MAPK)”, ERK7 (MAPK15), GSK3 alpha, GSK3 beta, HGK (MAP4K4), HSP27, JNK1 (MAPK8), JNK2 (MAPK9), JNK3 (MAPK10), KHS1 (MAP4K5), KHS2 (MAP4K3), LAMTOR3 (MAP2K1IP1), MAP2K4, MEK1 (MAP2K1), MEK2 (MAP2K2), MEK3 (MAP2K3), MEK5 (MAP2K5), MEK6 (MAP2K6), MEKK1 (MAP3K1), MEKK3 (MAP3K3), MEKK4 (MAP3K4), MINK (MAP4K6), MK2 (MAPKAPK2), MK3 (MAPKAPK3), MLK (MAP3K13), MLK1 (MAP3K9), MLK2 (MAP3K10), MLK3 (MAP3K11), MLK4 (MAP3KL4), MSK1 (RPS6KA5), mTOR, NF-kB p65, p38A (MAPK14), p38B (MAPK11), p38D (MAPK13), p38G (MAPK12), p44/42 MAPK, p53, p70S6K (RPS6KB1), p70S6K2 (RPS6KB2), PRAK (MAPKAPK5), RSK1 (RPS6KA1), RSK2 (RPS6KA3), RSK3 (RPS6KA2), RSK4 (RPS6KA6), RSKL1 (RPS6KC1), STAT1, TAB1 (MAP3K7IP1), TAK1 (MAP3K7)

The ELISA based MAPK Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Antibody Array - How It Works

Antibody Array User’s Guide

Antibody List

Array Map

GAL File  (To download, right click on the file name, then choose “Save target as”)

Material Safety Data Sheet (MSDS)

Frequently Asked Questions

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. You will receive raw images in tiff format.

Cost: Free of Charge

ADDITIONAL SERVICES

If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.

Cost: Free

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $150 per slide

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,240 per sample

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