Cytoskeleton Phospho Antibody Array

Cytoskeleton Phospho Antibody Array

Cytoskeleton Phospho Antibody Array features 141 highly specific antibodies related to cytoskeletal regulation. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PCP141

$500.00

Cytoskeleton Phospho Antibody Array

Cytoskeleton Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is designed for studying key proteins for cytoskeletal signaling and regulation, suitable for comparing normal samples to treated or diseased samples and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine and serine/threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening
  • Suitable samples: cell lysates; frozen or FFPE tissue lysates
  • Antibodies are covalently attached to 3D polymer coated glass slides
  • High specificity and affinity; low background
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 141 site-specific and phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 100%; Rat: 81%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months

 

Selected Publications

Rosenfeld S, Rich J, Mitotic Kinesin Eg5 Inhibiting Anticancer Agents, US Patent Application 20150352114

Woollard S, Li H, HIV-1 induces cytoskeletal alterations and Rac1 activation during monocyte-blood-brain barrier interactions: modulatory role of CCR5, Retrovirology 2014, 11:20

Ordering Information 
Catalog No Description Size Price
PCP141 Cytoskeleton Phospho Antibody Array 2 slides/pk $500
Additional Reagent Needed:

The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

Antibody Array - How It Works

 Questions? Visit our Frequently Asked Questions page.

Actin Pan (a/b/g) (Tyr55/53), Actin alpha-2/3, Actin-alpha1 sk muscle, ACTN1, Calmodulin (Thr79/Ser81), CaMK1-alpha (Thr177), CaMK1-beta, CaMK2 (Thr286), CaMK2 (Thr305), CaMK2-b/g/d (Thr287), CaMK4 (Thr196/200), CAMK5, Cofilin (Ser3), Cortactin (Tyr421), Cortactin (Tyr466), c-Raf (Ser296), c-Raf (Ser43), CrkII (Tyr221), CrkL (Tyr207), ERK1/2, ERK3 (Ser189), ERK8 (Thr175/Tyr177), Ezrin (Thr566), Ezrin (Tyr353), Ezrin (Tyr478), FAK (Ser910), FAK (Tyr397), FAK (Tyr407), FAK (Tyr576), FAK (Tyr861), FAK (Tyr925), Filamin A (Ser2152), GRB2 (Ser159), GTPase activating protein (Ser387), LIMK1 (Thr508), MEK1 (Ser217), MEK1 (Ser221), MEK1 (Thr291), MEK1 (Ser298), MEK1 (Thr286), MEK2 (Thr394), MEKKK1, MEKKK4, Merlin (Ser10), Merlin (Ser518), MKK3 (Ser189), MKK3/MAP2K3 (Thr222), MKK6 (Se207), MKK7/MAP2K7 (Ser271), MKK7/MAP2K7 (Thr275), Myosin regulatory light chain 2 (Ser18), NCK2, p130Cas (Tyr165), p130Cas (Tyr410), p44/42 MAPK (Thr202), p44/42 MAPK (Tyr204), Paxillin (Tyr118), Paxillin (Tyr31), PI3K-p85-alpha (Tyr607), PI3K-p85-a/g (Tyr467/Tyr199), PIP5K, PIP5K (Ser307), PKA CAT (Thr197), PKC alpha (Tyr657), PKC alpha/beta II (Thr638), PKC pan activation site, PLC beta (Ser1105), PLC beta3 (Ser537), Rac1/cdc42 (Ser71), Rho/Rac GEF (Ser885), Src (Ser75), Src (Tyr216), Src (Tyr418), Src (Tyr529), VASP (Ser157), VASP (Ser238), WASP (Tyr290), WAVE1 (Tyr125)

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

El-Haibi CP, Singh R, Antibody Microarray Analysis of Signaling Networks Regulated by Cxcl13 and Cxcr5 in Prostate Cancer. Journal of Proteomics and Bioinformatics 2012, 5(8): 177-184

Gomez JC, Doerschuk CM, Rac2 in murine pulmonary microvascular endothelial cells (ECs), FASEB Journal 2010, 24: 777.11

Li F, Chen T, Hu S, Superoxide Mediates Direct Current Electric Field-Induced Directional Migration of Glioma Cells through the Activation of AKT and ERK, PLoS ONE 2013, 8(4): e61195. doi:10.1371/journal.pone.0061195

Ranzato E, Martinotti S, Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: Comparison among different honeys, Wound Repair and Regeneration 2012, 20: 778–785

Rosenfeld S, Rich J, Mitotic Kinesin Eg5 Inhibiting Anticancer Agents, US Patent Application 20150352114

Woollard S, Li H, HIV-1 induces cytoskeletal alterations and Rac1 activation during monocyte-blood-brain barrier interactions: modulatory role of CCR5, Retrovirology 2014, 11:20

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,200 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $200 per slide

 

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