Wnt Signaling Phospho Antibody Array

Wnt Signaling Phospho Antibody Array

Wnt Signaling Phospho Antibody Array features 227 highly specific antibodies important to the Wnt/β-Catenin pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples in human, mouse, or rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PNT227

$650.00

Wnt Signaling Phospho Antibody Array

Wnt signaling has a direct association with cancer, type II diabetes, and neurodiseases. Signaling of the highly secreted glycolipoproteins in the Wnt family controls embryonic development and adult homeostasis by regulating β-Catenin’s accumulation in the cytoplasm. When Wnt signaling is absent, β-Catenin is targeted for ubiquitination and degraded by the destruction complex formed by Axin, APC, GSK3, PP2P, and CK-1α. As a result, β-Catenin cannot accumulate in the cytoplasm. When Wnt binds to Frizzled receptors and LRP5/6, Wnt signaling is turned on and disrupts the destruction complex’s function. Now β-Catenin accumulates, moves into the nucleus, and binds to TCF/LEF transcriptor factors to activate gene transcription.

Wnt Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine and serine/threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening
  • Suitable samples include: cell lysates; frozen or FFPE tissue lysates
  • Glass based array with high specificity and low background
  • Antibodies covalently attached to 3D polymer coated glass surface
  • Sensitive fluorescent detection
User’s Guide

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 227 site-specific and phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 92%; Rat: 80%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months

 

Selected Publications

Kogut MH, Arsenault RJ, A Role for the Non-Canonical Wnt-β-Catenin and TGF-β Signaling Pathways in the Induction of Tolerance during the Establishment of a Salmonella enterica Serovar Enteritidis Persistent Cecal Infection in Chickens, Front Vet Sci, 2015; 2: 33

Oldefest M, Düsterhöft S, Secreted Frizzled-related protein 3 (sFRP3)-mediated suppression of Interleukin-6 receptor release by A disintegrin and Metalloprotease 17 (ADAM17) is abrogated in the osteoarthritis-associated rare double variant of sFRP3Biochemical Journal, 2015 Jun 15;468(3):507-18

Ordering Information 
Catalog No Description Size Price
PNT227 Wnt Pathway Phospho Antibody Array 2 slides/pk $650
Additional Reagent Needed:

The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated  streptavidin.

Antibody Array - How It Works

 Questions? Visit our Frequently Asked Questions page.

 

AKT (Ser124), AKT (Ser246), AKT (Ser473), AKT (Thr308), AKT (Thr450), AKT (Thr72), AKT (Tyr326), AKT (Tyr474), AKT1 S1 (Thr246), AKT1/2/3 (Tyr315/316/312), AKT2 (Ser474), APC (Ser2054), APC1 (Ser355), APC1 (Ser688), CaMK2a (Thr286), CaMK2a/b/d (Thr305), CaMK2b/g/d (Thr287), Catenin a1 (Ser641), Catenin beta (Ser33), Catenin beta (Ser37), Catenin beta (Thr41/Ser45), Catenin beta (Tyr489), Catenin beta (Tyr654), Catenin delta1 (Tyr228), CKI-a (Tyr294), CKI-a (Tyr321), CKI-g 1/2/3 (Tyr263), CKII-a (Tyr255), CKII-b (Ser209), ERK3 (Ser189), ERK8 (Thr175/Tyr177), GPR120, GPR132, GPR150, GPR151, GPR152, GPR153, GPR160, GPR171, GPR173, GPR174, GPR175, GPR18, GSK3a (Ser21), GSK3a/b (Tyr216/279), GSK3b (Ser9), JNK1/2/3 (Thr183/Tyr185), MAP3K1 (Thr1402), MAP3K10, MAP3K11 (Ser674), MAP3K11 (Thr277/Ser281), MAP3K15, MAP3K3, MAP3K4, MAP3K6, MAP3K7 (Ser439), MAP3K71 (Thr187), MAP3K8 (Ser400), MAP3K9, MAP3KL4, MEK1 (Ser217), MEK1 (Ser221), MEK1 (Ser298), MEK1 (Thr286), MEK1 (Thr291), MEK2 (Thr394), MEKKK1, MEKKK4, mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), NFAT1 (Ser326), NFAT3 (Ser168/Ser170), NFAT3 (Ser676), NFAT4 (Ser165), NFAT5 (Ser1197), P38 MAPK (Thr179/Tyr181), P38 MAPK (Thr180), P38 MAPK (Tyr182), P38 MAPK (Tyr322), P44/42 MAPK, PDE4D (Ser190/53), PI3K-p85-a (Tyr607), PI3K-p85-a/g (Tyr467/Tyr199), PKA-a/b CAT (Thr197), PKA-R2-b (Ser113), PKC-a (Thr638), PKC-a (Tyr657), PKC-b (Ser661), PKC delta (Ser645), PKC delta (Thr505), PKC delta (Tyr313), PKC delta (Tyr52), PKC delta (Tyr64), PKC epsilon (Ser729), PKC pan (Thr497), PKC theta (Ser676), PKC theta (Ser695), PKC theta (Thr538), PKC zeta (Thr410), PKC zeta (Thr560), PKG2 (Ser126), PLC beta 3 (Ser1105), PLC beta 3 (Ser537), PLCG1 (Tyr1253), PLCG1 (Tyr771), PLCG1 (Tyr783), PLCG2 (Tyr1217), PLCG2 (Tyr753), PLCG2 (Tyr759), Rac1/cdc42 (Ser71), Rho/Rac GEF2 (Ser885), RhoA (Ser188), SAPK/JNK (Thr183), SAPK/JNK (Tyr185), Src (Ser75), Src (Tyr216), Src (Tyr418), Src (Tyr529), TAK1 (Thr184), Ubiquitin, VE-Cadherin (Tyr731), WNT1, WNT5A

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

 

de la Encarnación A, Alquéza C, Increased Wnt Signaling and Reduced Viability in a Neuronal Model of Progranulin-Deficient Frontotemporal Lobar DegenerationMol Neurobiol, First online: 17 December 2015

Kim, SY, Han YM, DUSP4 regulates neuronal differentiation and calcium homeostasis by modulating ERK1/2 phosphorylationStem Cells and Development 2015 Mar 15;24(6):686-700. doi: 10.1089/scd.2014.0434

Kogut MH, Arsenault RJ, A Role for the Non-Canonical Wnt-β-Catenin and TGF-β Signaling Pathways in the Induction of Tolerance during the Establishment of a Salmonella enterica Serovar Enteritidis Persistent Cecal Infection in Chickens, Frontiers in Veterinary Science, 2015; 2: 33

Oldefest M, Düsterhöft S, Secreted Frizzled-related protein 3 (sFRP3)-mediated suppression of Interleukin-6 receptor release by A disintegrin and Metalloprotease 17 (ADAM17) is abrogated in the osteoarthritis-associated rare double variant of sFRP3Biochemical Journal, 2015 Jun 15;468(3):507-18

Osborn D, Roccasecca R, Loss of FTO Antagonises Wnt Signaling and Leads to Developmental Defects Associated with Ciliopathies, PLoS ONE 2014 Feb 4;9(2):e87662. doi: 10.1371/journal.pone.0087662

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,350 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $250 per slide

 

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