T-Cell Receptor Phospho Antibody Array Assay Service

T-Cell Receptor Phospho Antibody Array Assay Service

Study T-Cell Receptor pathway with our TCR Signaling Phospho Antibody Array Assay Service, powered by Full Moon BioSystems’ advanced microarray platform. This high-throughput assay enables the simultaneous detection of over 213 site-specific and pan-specific antibodies, providing a robust overview of phosphorylation states of signaling proteins.

$1,520.00

Cat #: SEV03-PTC188

WHAT WE OFFER

We streamline your protein phosphorylation research by handling the entire protein profiling process using the T-Cell Phospho Antibody Array:

Sample Processing

Send your cell lysates or protein extracts to us, and our experienced technicians will take care of all sample preparation steps according to Full Moon BioSystems’ validated protocols.

Assay Execution

We perform the complete lysate preparation, labeling, incubation, and detection process using the T-Cell Phospho Antibody Array, ensuring high sensitivity and specificity for phosphorylated and total protein targets.

Imaging & Data Analysis

Our lab uses high-resolution microarray scanners and advance software tools to extract and analyze fluorescent signals from array images. Each spot is carefully examined to verify signal quality, consistency, and accuracy across the array.

This combined computational and expert-reviewed approach ensures high-confidence, reproducible results. We focus on delivering clean, reliable data that reflects true biological differences. This rigorous quality control process ensures that only high-confidence data are carried forward for interpretation.

Final Report Delivery

Upon completion of the assay and analysis, results are delivered in Excel format with clearly structured data. Each report includes:

  • Mean signal intensity of all replicate spots for each antibody

  • Coefficient of variation (CV) for replicate spots to assess signal consistency

  • Normalized signal intensities

  • Fold change calculations between control and. treatment samples

  • Ratios of phospho-antibody signals to their corresponding non-phospho antibody signals, providing insight into phosphorylation status relative to total protein levels

  • Sample Assay Results
APPLICATIONS
  • Protein phosphorylation profiling

  • Biomarker profiling

  • Cancer and drug target discovery

  • Signal pathway research

Technical Information

The ELISA based Phospho Explorer Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Antibody Array - How It Works

This array features 213 site-specific and phospho-specific antibodies from 16 commonly studies cell signaling pathways. Each antibody is printed with six replicates.

Antibody Reactivity: Human: 99% | Mouse: 82% | Rat: 55%

Selected Targets:

Protein TargetPhospho Site(s)ReactivityUniProt ID
AKT1Ser473Human, Mouse, RatP31749
CD3ζ (CD247)Tyr142Human, MouseP20963
ERK1Thr202Human, Mouse, RatP27361
IκBαSer32/Ser36Human, Mouse, RatP25963
JNK1Thr183/Tyr185Human, Mouse, RatP45983
LATTyr191Human, Mouse, RatO43561
LCKTyr505Human, MouseP06239
NFAT4Ser165Human, MouseO95644
p38 MAPKThr180Human, Mouse, RatQ16539
ZAP70Tyr319Human, MouseP43403

Ghosh D, Pham TD, Regulation of the BCR signalosome by the class II peptide editor, H2-M, affects the development and repertoire of innate-like B cells, Cell Rep. 2022 Jan 25;38(4):110200

Khameneh HJLeong KWK, The Inflammasome Adaptor ASC Intrinsically Limits CD4+ T-Cell Proliferation to Help Maintain Intestinal Homeostasis, Front Immunol. 2019; 10: 1566.

Sample Requirements
  • Cell and tissues
    • Cells: > 5 million cells
    • Tissues: > 75 mg
  • Lysates or protein extracts
    • Protein concentration: > 2 mg/mL recommended
    • Protein amount: > 400 ug
    • Compatible lysis buffer: mild, non-denaturing lysis, e.g., RIPA, T-per, M-per (avoid high salt/detergent concentrations)
  • Samples are stored at −80°C prior to shipping
  • Assay Service Guide (Includes detailed sample preparation and submission instructions and forms)
Packaging & Shipping
  • Samples must be frozen and shipped on dry ice in an insulated container
  • Include sufficient dry ice for 48+ hours of transit time. Arrange shipment to ship early in the week (Monday–Wednesday for domestic shipments; Monday for international shipments) via overnight or international priority service.
  • Email package tracking number to moc.o1749504546ibnoo1749504546mlluf1749504546@trop1749504546pus1749504546 to notify us of incoming shipments.
  • Documentation
    • A complete Sample Submission Form (MS WORD) placed inside your shipping container.
    • If submitting from outside the U.S., include required customs documents:

      • Commercial invoice
      • Shipper’s Declaration stating “Non-hazardous biological sample for research use only”. This declaration must be completed on your institution’s letterhead.

Ordering
Price: $1,520/sample

Note: The price listed is per sample per condition. Submitting both a control and a treatment sample counts as two samples.

 

U.S. Customers
International Customers
  • Prepayment Required: Payment must be made in advance by credit card or wire transfer.

  • Please contact us for a formal quote, invoice, and payment instructions.

  • We’ll guide you through the sample shipping process and required documentation.

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