WHAT WE OFFER
We simplify your protein phosphorylation research by taking care of protein profiling with Phospho Explorer Antibody Array—from start to finish:
Sample Processing
Send your cell lysates or protein extracts to us, and our experienced technicians will take care of all sample preparation steps according to Full Moon BioSystems’ validated protocols.
Assay Execution
We perform the complete lysate preparation, labeling, incubation, and detection process using the Phospho Explorer Array, ensuring high sensitivity and specificity for phosphorylated and total protein targets.
Imaging & Data Analysis
Our lab uses high-resolution microarray scanners and advance software tools to extract and analyze fluorescent signals from array images. Each spot is carefully examined to verify signal quality, consistency, and accuracy across the array.
This combined computational and expert-reviewed approach ensures high-confidence, reproducible results. We focus on delivering clean, reliable data that reflects true biological differences. This rigorous quality control process ensures that only high-confidence data are carried forward for interpretation.
Final Report Delivery
Upon completion of the assay and analysis, results are delivered in Excel format with clearly structured data. Each report includes:
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Mean signal intensity of all replicate spots for each antibody
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Coefficient of variation (CV) for replicate spots to assess signal consistency
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Normalized signal intensities
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Fold change calculations between control and. treatment samples
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Ratios of phospho-antibody signals to their corresponding non-phospho antibody signals, providing insight into phosphorylation status relative to total protein levels
- Sample Assay Results
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Protein phosphorylation profiling
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Biomarker profiling
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Cancer and drug target discovery
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Signal transduction research
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Kinase activity studies
The ELISA based Phospho Explorer Antibody Array platform involves four major steps:
- Protein extraction with non-denaturing lysis buffer
- Biotinylation of protein samples
- Incubation of labeled samples with antibody array
- Detection by dye conjugated streptavidin
Phospho Explorer Array features 1318 antibodies targeting key proteins involved in phosphorylation-dependent signaling.
Antibody Reactivity: Human: 99% | Mouse: 92% | Rat: 66%
Selected targets:
| Protein Target | Phospho Site(s) | Reactivity | UniProt ID | Key Pathway / Function |
|---|---|---|---|---|
| AKT1 | Ser473 | Human, Mouse, Rat | P31749 | PI3K/AKT signaling |
| Cyclin D1 | Thr286 | Human, Mouse, Rat | P24385 | Cell cycle regulation |
| ERK1 | Thr202 | Human, Mouse, Rat | P27361 | MAPK signaling |
| EGFR | Tyr1997 | Human, Mouse, Rat | P00533 | PI3K/AKT signaling |
| GSK-3β | Ser9 | Human, Mouse, Rat | P49841 | Wnt signaling / glycogen metabolism |
| JNK1 | Thr183 | Human, Mouse, Rat | P45983 | Apoptosis / stress response |
| mTOR | Ser2448 | Human, Mouse, Rat | P42345 | mTOR signaling / cell growth |
| NF-κB p65 | Ser536 | Human, Mouse | Q04206 | Inflammation / transcriptional control |
| p38 MAPK | Thr180 | Human, Mouse, Rat | Q16539 | Stress response |
| SRC | Tyr418 | Human, Mouse, Rat | P12931 | Tyrosine kinase / cell adhesion |
| STAT3 | Tyr705 | Human, Mouse, Rat | P40763 | JAK/STAT pathway |
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- Cell and tissues
- Cells: > 5 million cells
- Tissues: > 75 mg
- Lysates or protein extracts
- Protein concentration: > 2 mg/mL recommended
- Protein amount: > 400 ug
- Compatible lysis buffer: mild, non-denaturing lysis, e.g., RIPA, T-per, M-per (avoid high salt/detergent concentrations)
- Samples are stored at −80°C prior to shipping
- Assay Service Guide (Includes detailed sample preparation and submission instructions and forms)
- Samples must be frozen and shipped on dry ice in an insulated container
- Include sufficient dry ice for 48+ hours of transit time. Arrange shipment to ship early in the week (Monday–Wednesday for domestic shipments; Monday for international shipments) via overnight or international priority service.
- Email package tracking number to moc.o1749504182ibnoo1749504182mlluf1749504182@trop1749504182pus1749504182 to notify us of incoming shipments.
- Documentation
- A complete Sample Submission Form (MS WORD) placed inside your shipping container.
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If submitting from outside the U.S., include required customs documents:
- Commercial invoice
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Shipper’s Declaration stating “Non-hazardous biological sample for research use only”. This declaration must be completed on your institution’s letterhead.
Price: $2,310/sample
Note: The price listed is per sample per condition. Submitting both a control and a treatment sample counts as two samples.
U.S. Customers
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Online Orders: credit card only
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Purchase Orders (POs): We accept institutional or corporate POs. Email your PO to moc.o1749504182ibnoo1749504182mlluf1749504182@sred1749504182ro1749504182.
International Customers
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Prepayment Required: Payment must be made in advance by credit card or wire transfer.
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Please contact us for a formal quote, invoice, and payment instructions.
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We’ll guide you through the sample shipping process and required documentation.
