Cell Signaling Phospho Antibody Array Assay Service

Cell Signaling Phospho Antibody Array Assay Service

Discover comprehensive insights into cell signaling dynamics with our Cell Signaling Phospho Antibody Array Assay Service, powered by Full Moon BioSystems’ high-performance microarray platform. This assay enables the simultaneous detection of hundreds of site-specific phosphorylated proteins, providing a broad and detailed overview of critical signaling pathways.

$1,730.00

Cat #: SEV03-PCS300

WHAT WE OFFER

We simplify your protein phosphorylation research by taking care of protein profiling with Cell Signaling Phospho Antibody Array—from start to finish:

Sample Processing

Send your cell lysates or protein extracts to us, and our experienced technicians will take care of all sample preparation steps according to Full Moon BioSystems’ validated protocols.

Assay Execution

We perform the complete lysate preparation, labeling, incubation, and detection process using the Cell Signaling Phospho Antibody Array, ensuring high sensitivity and specificity for phosphorylated and total protein targets.

Imaging & Data Analysis

Our lab uses high-resolution microarray scanners and advance software tools to extract and analyze fluorescent signals from array images. Each spot is carefully examined to verify signal quality, consistency, and accuracy across the array.

This combined computational and expert-reviewed approach ensures high-confidence, reproducible results. We focus on delivering clean, reliable data that reflects true biological differences. This rigorous quality control process ensures that only high-confidence data are carried forward for interpretation.

Final Report Delivery

Upon completion of the assay and analysis, results are delivered in Excel format with clearly structured data. Each report includes:

  • Mean signal intensity of all replicate spots for each antibody

  • Coefficient of variation (CV) for replicate spots to assess signal consistency

  • Normalized signal intensities

  • Fold change calculations between control and. treatment samples

  • Ratios of phospho-antibody signals to their corresponding non-phospho antibody signals, providing insight into phosphorylation status relative to total protein levels

  • Sample Assay Results
APPLICATIONS
  • Protein phosphorylation profiling

  • Biomarker profiling

  • Cancer and drug target discovery

  • Signal pathway research

Technical Information

The ELISA based Phospho Explorer Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Antibody Array - How It Works

This array features 304 site-specific and phospho-specific antibodies from 16 commonly studies cell signaling pathways. Each antibody is printed with six replicates.

Antibody Reactivity: Human: 99% | Mouse: 87% | Rat: 66%

PI3K/AKT
AKT1, AKT2, BAD, BCL2, CASP9, CCND1, CCNE1, CDK2, CKDN1A, CHUK, CREB1, EGFR1, EIF4E, EIF4EBP1, ERBB2, FOXO3, GSK3B, HSP90AB1, IGF1R, IKBKB, IKBKG, IL4R, IRS1, ITGB3, JAK1, JAK2, KDR, MAP2K1, MDM2, MET, MTOR, MYC, NFKB1, NOS3, NTRK2, PDGFRB, PDK1, PPP2CA, PRKAA1, PTEN, PTK2, RAF1, RELA, RPS6KB1, STK11, SYK, TP53, TSC2, YWHAQ

AMPK
ACACA, AKT1, AKT1S1, AKT2, CCND1, CREB1, EEF2, EEF2K, EIF4EBP1, FOXO1, FOXO3, IFG1R, IRS1, MAP3K7, MTOR, PDPK1, PPARG, PPP2CA, PRKAA1, RPS6KB1, SREBF1, STK11, TSC2

Apoptosis
ACTB, ACTG1, AKT1, AKT2, BAD, BAX, BCL2, BID, CASP3, CASP8, CASP9, CHUK, EIF2S1, FADD, FOS, IKBKB, IKBKG, LMNA, MAP2K1, MAP3K5, MAPK9, NFKB1, NFKB1A, PDPK1, RAF1, RELA, TP53

Autophagy
AKT1, AKT1S1, AKT2, BAD, BCL2, BECN1, EIF2S1, IGF1R, IRS1, MAP2K1, MAP3K7, MAPK9, MTOR, PDPK1, PPP2CA, PRKAA1, PTEN, RAF1, RPS6KB1, STK11, TSC2

Cell Cycle
ABL1, CCND1, CCNE1, CDC25C, CDK2, CDKNIA, CHEK1, CHEK2, GSK3B, HDAC1, HDAC2, MDM2, MYC, PLK1, RB1, SMAD2, SMAD3, TP53, YWHAQ

ErbB
ABL1, AKT1, AKT2, BAD, BRAF, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CBL, CDKN1A, CRK, EGFR, EIF4EBP1, ELK1, ERBB2, GAB1, GSK3B, MAP2K1, MAP2K4, MAPK9, MTOR, MYC, PAK1, PLCG1, PTK2, RAF1, RPS6KB1, SHC1, SRC, STAT5A, STAT5B ,

Focal Adhesion
ACTB, ACTG1, AKT1, AKT2, BAD, BCL2, BRAF, CCND1, CRK, CTNNB1, EGFR, ELK1, ERBB2, GSK3B, IGF1R, ITGB3, KDR, MAP2K1, MAKP9, MET, PAK1, PDGFRB, PDPK1, PPP1CA, PTEN, PTK2, RAF1, RASGRF1, SHC1, SRC, VASP

Insulin
ACACA, AKT1, AKT2, BAD, BRAF, CALM1, CBL, CRK, EIF4E, EIF4EBP1, ELK1, FOXO1, GSK3B, IKBKB, IRS1, MAK2K1, MAPK9, MTOR, PDPK1, PPP1CA, PRKAA1, RAF1, RPS6KB1, SHC1, SREBF1, TSC2 ,

JAK-STAT
AKT1, AKT2, BCL2, CCND1, CDKN1A, EGFR, IFNGR1, IL10RA, IL4R, JAK1, JAK2, MTOR, MYC, PDGFRB, PTPN11, RAF1, STAT1, STAT2, STAT3, STAT4, STAT5A, STAT5B, STAT6

MAPK
AKT1, AKT2, BRAF, CHUK, CRK, EGFR, ELK1, ERBB2, FOS, HSPB1, IGR1R, IKBKG, KDR, MAP2K1, MAP2K4, MAP3K5, MAP3K7, MAPK14, MAPK9, MAPT, MET, MYC, NFKB1, NTRK2, PAK1, PDFGRB, RAF1, RASGRF1, RELA, RELB, RPS6KA1, RPS6KA2, RPS6KA3, RPS6KA5, SRF, TP53, CASP3

mTOR
AKT1, AKT1S1, AKT2, BRAF, CHUK, EIF4E, EIF4EBP1, GSK3B, IFG1R, IKBKB, IRS1, MAP2K1, MTOR, PDPK1, PRKAA1, PTEN, RAF1, RPS6KA1, RPS6KA2, RPS6KA3, RPS6KB1, STK11, TSC2, WNT1

NF-kappa B
BCL2, BLNK, CHUK, IKBKB, IKBKG, LCK, LYN, MAP3K7, NFKB1, NFKB1A, PLCG1, RELA, RELB, SYK, ZAP70

p53
BAX, BCL2, BID, CASP8, CASP9, CCND1, CCNE1, CDK2, CDKNIA, CHEK1, CHEK2, MDM2, PTEN, TP53, TP73, TSC2, CASP3, CASP8

Ras
ABL1, AKT1, AKT2, BAD, CALM1, CHUK, EGFR, ELK1, GAB1, GAB2, GRIN1, IGF1R, IKBKB, IKBKG, KDR, MAP2K1, MAPK9, MET, NFKB1, PAK1, PDFGRB, PLCG1, PTPN11, RAF1, RASGRF1, RELA, SHC1, ZAP70

TGF-beta
MYC, PPP2CA, RPS6KB1, SMAD1, SMAD2, SMAD3, TGFBR1, TGFBR2

VEGF
AKT1, AKT2, BAD, CASP9, HSPB1, MAP2K1, MAPK14, NOS3, PLCG1, PTK2, RAF1, SRC, VEGFR2

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Sample Requirements
  • Cell and tissues
    • Cells: > 5 million cells
    • Tissues: > 75 mg
  • Lysates or protein extracts
    • Protein concentration: > 2 mg/mL recommended
    • Protein amount: > 400 ug
    • Compatible lysis buffer: mild, non-denaturing lysis, e.g., RIPA, T-per, M-per (avoid high salt/detergent concentrations)
  • Samples are stored at −80°C prior to shipping
  • Assay Service Guide (Includes detailed sample preparation and submission instructions and forms)
Packaging & Shipping
  • Samples must be frozen and shipped on dry ice in an insulated container
  • Include sufficient dry ice for 48+ hours of transit time. Arrange shipment to ship early in the week (Monday–Wednesday for domestic shipments; Monday for international shipments) via overnight or international priority service.
  • Email package tracking number to moc.o1749492375ibnoo1749492375mlluf1749492375@trop1749492375pus1749492375 to notify us of incoming shipments.
  • Documentation
    • A complete Sample Submission Form (MS WORD) placed inside your shipping container.
    • If submitting from outside the U.S., include required customs documents:

      • Commercial invoice
      • Shipper’s Declaration stating “Non-hazardous biological sample for research use only”. This declaration must be completed on your institution’s letterhead.

Ordering
Price: $1,730/sample

Note: The price listed is per sample per condition. Submitting both a control and a treatment sample counts as two samples.

 

U.S. Customers
International Customers
  • Prepayment Required: Payment must be made in advance by credit card or wire transfer.

  • Please contact us for a formal quote, invoice, and payment instructions.

  • We’ll guide you through the sample shipping process and required documentation.

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