ANTIBODY ARRAY ASSAY SERVICE
Send your samples to our lab for the complete antibody array assay service. Researchers can select from a wide range of antibody microarrays to study protein changes and phosphorylation events. Once we receive your samples, whether they are cells, tissues or serum, our experienced scientists will perform complete antibody array assays. This convenient assay service offers quick turnaround, accurate and reliable results, saving you time and resources!
SERVICES INCLUDED
- Protein extraction and labeling
- Assay condition optimization
- Conjugation of biotinylated sample to to antibody array
- Detection using Cy3-streptavidin
- Array scanning
- Image quantification and data analysis
RESULTS
- Turnaround time: 5-10 business days for 2-5 samples
- Assay data provided in Excel format
- Assay protocol and data interpretation guidelines
- Results are delivered to investigators by email
- Sample Assay Results
WHY CHOOSE US
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Expert team with 20+ years of experience in array-based proteomics
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Consistent performance; High sensitivity
- Quick turnaround
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Trusted by researchers in academia and industry
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Supported by peer-reviewed publications
ASSAY PRINCIPLE
1) Protein extracts are prepared with non-denaturing lysis buffer. Suitable Samples include cells, tissues, lysates, culture media, serum, FFPE
2) Proteins are labeled with biotin.
3) Biotinylated samples are incubated with antibody array.
4) Detection by dye conjugated streptavidin.
SAMPLE PREPARATION AND SUBMISSION
Sample Submission
- Pack samples with sufficient dry ice.
- Send by FedEx/UPS overnight or international priority service.
- Forward submission and package tracking details to moc.o1749479890ibnoo1749479890mlluf1749479890@trop1749479890pus1749479890.
- Forms required:
Sample Requirements
- Cells: 5 – 10 million
- Tissues: > 75 mg
- Serum: 5-10 uL
- Lysates:
- Protein amount: > 400 ug
- Preferred concentration: > 2 ug/uL
- Lysis buffer: mild, non-denaturing lysis buffer
ARRAY SELECTION
| Array Name | Catalog No. | Price (Per Sample) |
|---|---|---|
| Apoptosis Antibody Array | SEV04-APP069 | $1,240 |
| Cancer BioMarker Antibody Array | SEV07-SCB200 | $1,520 |
| Cell Cycle Antibody Array | SEV04-ACC058 | $1,240 |
| Cytokine Profiling Antibody Array | SEV07-SCK100 | $1,730 |
| Explorer Antibody Array | SEV04-ASB600 | $1,730 |
| Inflammation Antibody Array | SEV07-SMA090 | $1,240 |
| Kinase Antibody Array | SEV05-AVK276 | $1,730 |
| MAPK Signaling Antibody Array | SEV07-SMK060 | $1,240 |
| Signal Transduction Antibody Array | SEV04-AST160 | $1,470 |
| Signaling Explorer Array | SEV06-SET100 | $2,310 |
HOW TO ORDER
US Customers
- Request your purchasing department to send a purchase order by email to .moc.1749479890oibno1749479890omllu1749479890f@sel1749479890as1749479890
- Credit card orders: please call 408.737.2875 to place an order.
- Ship your samples to Full Moon BioSystems. Please refer to the Antibody Array Service Guide for sample preparation and shipping instructions.
International Customers
- Contact us to obtain a proforma invoice.
- Send payment by electronic bank transfer or by check.
- Confirm receipt of payment with us.
- Ship your samples to Full Moon BioSystems. Please refer to the Antibody Array Service Guide for sample preparation and shipping instructions.
FREQUENTLY ASKED QUESTIONS
Q: What types of samples are compatible with this service?
A: We accept cell pellets, whole cell lysates, frozen tissues, tissue lysates, and serum/plasma (with optimization).
Q: How much sample do I need to provide?
A: We recommend submitting:
Cells: 5 -1 0 million
Tissues: 75mg or more
Lysates: >400ug of protein; concentration: >2 mg/mL
Q: What lysis buffer should I use to prepare lysates?
A: RIPA buffer works well for cells and tissues. Avoid strong detergents like SDS, which can interfere with labeling and hybridization. If SDS or Tris is included in the buffer, ensure SDS < 0.1%, Tris < 50mM. Other compatible lysis buffers include T-per Protein Extraction Reagent and M-per Protein Extraction Reagent. To preserve the protein, phosphatase inhibitors and protease inhibitors are recommended.
Q: Can I use mouse or rat samples?
A: Yes. Many of the antibodies on phosphorylation antibody arrays are cross-reactive with mouse and rat proteins. Review the antibody list or contact us for help verifying target compatibility.
Q: What’s the turnaround time for the assay service?
A: For 2 to 4 samples, our standard turnaround is 5 – 10 business days from the time we receive your samples and confirm the order. For more samples, please contact us for details.
Q: What data will I receive?
A: You will receive assay results in an Excel file. Here is a set of sample assay results.
Q: How should I ship my samples?
A: All samples must be shipped on dry ice. Include a completed sample submission form and notify us in advance to coordinate receipt.
More questions? Call 408.737.2875, email, or complete the form below.