ANTIBODY ARRAY ASSAY SERVICE
Take advantage of our complete antibody array assay service designed to deliver reliable, high-quality data with minimal effort on your end. Simply ship your samples to our laboratory, and our experienced scientists will handle the rest.
We offer a broad selection of antibody microarrays to help researchers analyze protein expression changes and phosphorylation events across multiple pathways. Whether your samples include cells, tissues, serum, or saliva samples, our team performs the entire workflow, from sample processing and array hybridization to data acquisition and analysis.
WHY CHOOSE US
- 20+ Years of Expertise – Extensive experience in array-based proteomics delivering reliable, high-quality results.
- Consistent Performance & High Sensitivity – Optimized platforms ensure consistent, accurate data you can trust.
- Quick Turnaround – Efficient workflows to keep your research on schedule.
- Trusted & Publication Supported – Preferred by academic and industry researchers, backed by peer-reviewed publications.
SERVICES INCLUDED
- Protein extraction and biotin labeling
- Assay condition optimization to maximize specificity and signal-to-noise ratio
- Target protein capture via antigen–antibody interactions on the antibody microarray
- Fluorescent detection using Cy3-conjugated streptavidin
- High-resolution microarray scanning
- Image processing, quantification, and data analysis
RESULTS
- Turnaround time: 5-10 business days for 2-5 samples (from receipt of qualified samples)
- Assay results provided in Excel format, including raw signal intensities and normalized data
- Assay protocol and data interpretation guidelines included
- Final results and supporting documents sent electronically to investigators via email
- Sample Assay Results
ASSAY PRINCIPLE
- Protein Extraction
Protein extracts are prepared using a non-denaturing lysis buffer to preserve native protein structure and biological activity. Suitable sample types include cells, tissues, lysates, culture media, saliva, serum, and FFPE samples. - Biotin Labeling
Extracted proteins are labeled with biotin, enabling sensitive and specific downstream detection while maintaining antigen–antibody interactions. - Target Protein Binding
Biotinylated samples are applied to the antibody array, where target proteins bind specifically to their corresponding immobilized capture antibodies. - Fluorescent Detection
Bound proteins are detected using dye-conjugated streptavidin, generating fluorescence signals that reflect relative protein expression levels for qualitative or semi-quantitative analysis.
SAMPLE PREPARATION AND SUBMISSION
Sample Requirements
- Cells: 5 – 10 million
- Tissues: > 75 mg
- Serum: 5-10 uL
- Lysates:
- Protein amount: > 400 ug
- Preferred concentration: > 2 ug/uL
- Lysis buffer: mild, non-denaturing lysis buffer
Sample Submission
- Pack samples with sufficient dry ice.
- Send by FedEx/UPS overnight or international priority service.
- Forward submission and package tracking details to moc.o1772771260ibnoo1772771260mlluf1772771260@trop1772771260pus1772771260.
- Forms required:
Download Assay Service Guide
ARRAY SELECTION
Phosphorylation Profiling Arrays
Total Protein Profiling Arrays
| Array Name | Catalog No. | Price (Per Sample) |
|---|---|---|
| Apoptosis Antibody Array | SEV04-APP069 | $1,240 |
| Cancer BioMarker Antibody Array | SEV07-SCB200 | $1,520 |
| Cell Cycle Antibody Array | SEV04-ACC058 | $1,240 |
| Cytokine Profiling Antibody Array | SEV07-SCK100 | $1,730 |
| Explorer Antibody Array | SEV04-ASB600 | $1,730 |
| Inflammation Antibody Array | SEV07-SMA090 | $1,240 |
| Kinase Antibody Array | SEV05-AVK276 | $1,730 |
| MAPK Signaling Antibody Array | SEV07-SMK060 | $1,240 |
| Signal Transduction Antibody Array | SEV04-AST160 | $1,470 |
| Signaling Explorer Array | SEV06-SET100 | $2,310 |
HOW TO ORDER
US Customers
- Request your purchasing department to send a purchase order by email to .moc.1772771260oibno1772771260omllu1772771260f@sel1772771260as1772771260
- Credit card orders: please call 408.737.2875 to place an order.
- Ship your samples to Full Moon BioSystems. Please refer to the Antibody Array Service Guide for sample preparation and shipping instructions.
International Customers
- Contact us to obtain a proforma invoice.
- Send payment by electronic bank transfer or by check.
- Confirm receipt of payment with us.
- Ship your samples to Full Moon BioSystems. Please refer to the Antibody Array Service Guide for sample preparation and shipping instructions.
FREQUENTLY ASKED QUESTIONS
Q: What types of samples are compatible with this service?
A: We accept cell pellets, whole cell lysates, frozen tissues, tissue lysates, and serum/plasma (with optimization).
Q: How much sample do I need to provide?
A: We recommend submitting:
Cells: 5 -1 0 million
Tissues: 75mg or more
Lysates: >400ug of protein; concentration: >2 mg/mL
Q: What lysis buffer should I use to prepare lysates?
A: RIPA buffer works well for cells and tissues. Avoid strong detergents like SDS, which can interfere with labeling and hybridization. If SDS or Tris is included in the buffer, ensure SDS < 0.1%, Tris < 50mM. Other compatible lysis buffers include T-per Protein Extraction Reagent and M-per Protein Extraction Reagent. To preserve the protein, phosphatase inhibitors and protease inhibitors are recommended.
Q: Can I use mouse or rat samples?
A: Yes. Many of the antibodies on phosphorylation antibody arrays are cross-reactive with mouse and rat proteins. Review the antibody list or contact us for help verifying target compatibility.
Q: What’s the turnaround time for the assay service?
A: For 2 to 4 samples, our standard turnaround is 5 – 10 business days from the time we receive your samples and confirm the order. For more samples, please contact us for details.
Q: What data will I receive?
A: You will receive assay results in an Excel file. Here is a set of sample assay results.
Q: How should I ship my samples?
A: All samples must be shipped on dry ice. Include a completed sample submission form and notify us in advance to coordinate receipt.
More questions? Call 408.737.2875, email, or complete the form below.