MAPK Phospho Antibody Array

MAPK Phospho Antibody Array

MAPK Pathway Phospho Antibody Array features 185 highly specific antibodies related to the MAPK pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog No: PMK185

$670.00

MAPK Pathway Phospho Antibody Array

MAPK Pathway Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine phosphorylation and serine/threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening with 185 antibodies
  • Antibodies covalently immobilized on 3D polymer coated glass slide
  • Fluorescent detection
Specifications
Product Size:2 array slides per package for analyzing two samples (untreated vs. treated)
Featured Antibodies:185 site-specific and phospho-specific antibodies; 6 replicates per antibody
Reactivity:Human: 100% | Mouse: 85%  Rat: 71%
Suitable Sample Type:Cell lysate | Tissue lysate
Detection Method:Fluorescence | Compatible Scanners
Internal Controls:Positive controls: beta-actin | GAPDH | Negative controls
Slide dimensions: 76 x 25 x 1 mm
Storage Condition:4°C for 6 months

 

PRODUCT DETAILS

4E-BP1(Thr36), 4E-BP1(Thr45), ASK1(Ser83), ASK1(Ser966), ATF2(Ser112 or 94), ATF2(Ser62 or 44), ATF2(Thr69 or 51), ATF2(Thr71 or 53), ATF2(Thr73 or 55), ATF4(Ser245), c-Jun(Ser243), c-Jun(Ser63), c-Jun(Ser73), c-Jun(Thr239), c-Jun(Thr91), c-Jun(Thr93), c-Jun(Tyr170), c-Kit(Tyr721), CREB(Ser129), CREB(Ser133), eIF2 alpha(Ser51), elF4E(Ser209), Elk1(Ser383), Elk1(Ser389), Elk1(Thr417), Estrogen Receptor-alpha (Ser104), Estrogen Receptor-alpha (Ser106), Estrogen Receptor-alpha (Ser118), Estrogen Receptor-alpha (Ser167), FAK(Tyr861), FAK(Tyr925), Histone H2A.X(Ser139), Histone H3.1(Ser10), HSF1(Ser303), HSP27(Ser15), HSP27(Ser78), HSP27(Ser82), IRS-1(Ser307), IRS-1(Ser312), IRS-1(Ser636), IRS-1(Ser639), JunB(Ser259), JunB(Ser79), JunD(Ser255), MEF2A(Thr312), MEF2A(Thr319), MEK1(Ser217), MEK1(Ser221), MEK1(Thr291), MEK2(Thr394), Met(Tyr1234), Met(Tyr1349), MKK3(Ser189), Myc(Ser373), Myc(Thr358), Myc(Thr58), P38 MAPK(Thr180), P38 MAPK(Tyr182), p44/42 MAP Kinase?Phospho-Thr202), p44/42 MAP Kinase(Tyr204), p53(Ser15), p53(Ser315), p53(Ser33), p53(Ser37), p53(Ser46), p53(Ser6), p53(Ser9), p53(Thr18), PKC delta(Ser645), PKC theta(Ser676), Pyk2(Tyr402), Raf1(Ser259), Raf1(Ser338), SAPK/JNK(Thr183), SAPK/JNK(Tyr185), SEK1/MKK4(Ser80), SEK1/MKK4(Thr261), Src(Tyr418), Src(Tyr529), Stathmin 1(Ser15), Stathmin 1(Ser24), Stathmin 1(Ser37), Tau(Ser214), Tau(Ser235), Tau(Ser262), Tau(Ser356), Tau(Ser396), Tau(Ser404), Tau(Ser422), Tau(Thr181), Tau(Thr205), Tau(Thr212), Tau(Thr231)

The ELISA based MAPK Pathway Phospho Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Antibody Array Assay Principle

Antibody Array User’s Guide

Antibody List

Array Map

GAL File  (To download, right click on the file name, then choose “Save target as”)

Material Safety Data Sheet (MSDS)

Frequently Asked Questions

Feng Y, He F, The Role of GOLPH3L in the Prognosis and NACT response in Cervical Cancer, J Cancer 2017; 8(3):443-454

Genebai N, Ahmad S, Genetic inhibition of JNK3 ameliorates spinal muscular atrophy, Hum Mol Genet, 2015 Sep 30. pii:ddv401

Rao W, Li H, OVA66 Increases Cell Growth, Invasion, and Survival via Regulation of IGF-1R-MAPK Signaling in Human Cancer Cells, Carcinogenesis2014 35(7):1573-81 [PubMed]

Jin L, Li D, p90 RSK2 Mediates Antianoikis Signals by both Transcription-Dependent and -Independent Mechanisms, Mol Cell Biol, 2013 33(13):2574-85

Kiso-Farne K, Tsuruyama T, Epidermal growth factor receptor cascade prioritizes the maximization of signal transduction, Sci Rep. 2022 Oct 10;12(1):16950

Tsuruyama T, Kullback–Leibler Divergence of an Open-Queuing Network of a Cell-Signal-Transduction Cascade, Entropy 2023, 25(2):326; https://doi.org/10.3390/e25020326

Yu Y, Richardson DR, Cellular iron depletion stimulates the JNK and p38 MAPK signaling transduction pathways, dissociation of ASK1-thioredoxin, and activation of ASK1, J Biol Chem, 2011 286(17):15413-27

Raharijaona M, Le Pennec S, PGC-1-Related Coactivator Modulates Mitochondrial-Nuclear Crosstalk through Endogenous Nitric Oxide in a Cellular Model of Oncocytic Thyroid Tumours, PLoS ONE, 2009 4(11):e7964

ADDITIONAL SERVICES

If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.

Cost: Free

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $200 per slide

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,470 per sample

You may also like…