WHAT WE OFFER
Streamline your research by managing the entire Explorer Antibody Array workflow—from sample to results.
Sample Processing
Send your cell lysates or protein extracts to us, and our experienced technicians will take care of all sample preparation steps according to Full Moon BioSystems’ validated protocols, ensuring consistency and compatibility with the Signaling Explorer platform.
Assay Execution
We perform the complete lysate preparation, labeling, incubation, and detection process using the Explorer Antibody Array. This array enables high-throughput profiling of total proteins involved in key cellular signaling pathways.
Imaging & Data Analysis
Our lab uses high-resolution microarray scanners and advance software tools to extract and analyze fluorescent signals from array images. Each spot is carefully examined to verify signal quality, consistency, and accuracy across the array.
Our hybrid approach, computational quantification plus expert-reviewed, ensures high-confidence, reproducible results. We focus on delivering clean, reliable data that reflects true biological differences. This rigorous quality control process ensures that only high-confidence data are carried forward for interpretation.
Final Report Delivery
Upon completion of the assay and analysis, results are delivered in Excel format with clearly structured data. Each report includes:
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Mean signal intensity of all replicate spots for each antibody
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Coefficient of variation (CV) for replicate spots to assess signal consistency
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Normalized signal intensities
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Fold change calculations between control and. treatment samples
- Sample Assay Results
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Biomarker profiling
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Cancer and drug target discovery
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Signal transduction research
- Mechanism of action studies
The ELISA based Phospho Explorer Antibody Array platform involves four major steps:
- Protein extraction with non-denaturing lysis buffer
- Biotinylation of protein samples
- Incubation of labeled samples with antibody array
- Detection by dye conjugated streptavidin
Explorer Antibody Array features 656 antibodies, each in duplicate format, targeting a wide variety of signaling pathways
Antibody Reactivity: Human
Selected targets :
| Antibody Target | Function / Role |
|---|---|
| Acinus | Apoptosis-related nuclear protein |
| Activin Receptor Type II | Growth factor receptor |
| AIF | Apoptosis-inducing mitochondrial flavoprotein |
| AIF (Apoptosis-Inducing Factor) | Mitochondrial apoptosis mediator |
| Alkaline Phosphatase | Enzyme marker |
| Alkaline phosphatase | Tissue enzyme implicated in bone/liver function |
| Alpha‑fetoprotein (AFP) | Liver cancer biomarker |
| Amyloid A, Amyloid A4, Amyloid β | Proteins associated with amyloid-related diseases |
| Bcl‑2, Bcl‑XL, Bax, Bak, Bim | Regulators of apoptosis pathways |
| Caspase‑1, -2, -3, -5, -6, -7, -8, -9 | Initiator & executioner caspases involved in programmed cell death |
| CD1, CD1a, CD1b, CD2, CD3ζ, CD4, CD5, CD6, CD8, CD9, CD10, CD100 | Immune-cell surface markers |
Bagnis, A, Izzotti, A, Aqueous humor oxidative stress proteomic levels in primary open angle glaucoma, Experimental Eye Research, 2012, 103:55–62
Burcham PC, Raso A, Airborne Acrolein Induces Keratin-8 (Ser-73) Hyperphosphorylation and Intermediate Filament Ubiquitination in Bronchiolar Lung Cell Monolayers, Toxicology May 7 2014, 319:44-52
Guamanova NG, Vasilyev DK, Application of an antibody microarray for serum protein profiling of coronary artery stenosis, Biochem Biophys Res Commun, 2022, 631:55-63
, Visible red and infrared light alters gene expression in human marrow stromal fibroblast cells, Orthodontics & Craniofacial Research 2015; 18(Suppl.1): 50–61
Fan Q, Zhou J, Chip-based serum proteomics approach to reveal the potential protein markers in the sub-acute stroke patients receiving the treatment of Ginkgo Diterpene Lactone Meglumine Injection, J Ethnopharmacol. 2020 May 12;112964. doi: 10.1016/j.jep.2020.11296
Klimushina MV, Gumanova NG, Direct labeling of serum proteins by fluorescent dye for antibody microarray, Biochem Biophys Res Commun 2017; 486(3):824-826
Lee MS, Kim JH, Prognostic Significance of CREB-Binding Protein and CD81 Expression in Primary High Grade Non-Muscle Invasive Bladder Cancer: Identification of Novel Biomarkers for Bladder Cancer Using Antibody Microarray, PLOS One, 2015 10(4):e0125405
Moon, KM, Park, Y, The Effect of Secretory Factors of Adipose-Derived Stem Cells on Human Keratinocytes, Intl J of Mol Sci 2012, 13(1):1239-1257
Quistad SD, Stotland A, Evolution of TNF-induced Apoptosis Reveals 550 My of Functional Conservation, PNAS 2014 111(26) 9567-9572
Ravishankar P, Dail A, Tears as the Next Diagnostic Biofluid: A Comparative Study between Ocular Fluid and Blood, Appl. Sci. 2022. 12(6):2884; https://doi.org/10.3390/app12062884
Siddique A, Ebrahim H, (−)-Oleocanthal Combined with Lapatinib Treatment Synergized against HER-2 Positive Breast Cancer In Vitro and In Vivo, Nutrients 2019; 11(2): 412
Yang T, Yao H, Effects of Lovastatin on MDA-MB-231 Breast Cancer Cells: An Antibody Microarray Analysis, Journal of Cancer, 2016; 7(2): 192-199
Yin SJ, Park D, An RNA interference based study for the role of ALDH1 in keratinocytes: DNA microarray, antibody–chip array and bioinformatics approaches, Process Biochem, 2014 49(10): 1612–1621
Zupancic K, Blejec A, Identification of plasma biomarker candidates in glioblastoma using an antibody-array-based proteomic approach, Radiology and Oncology, 2014, 48(3), 257–266
- Cell and tissues
- Cells: > 5 million cells
- Tissues: > 75 mg
- Lysates or protein extracts
- Protein concentration: > 2 mg/mL recommended
- Protein amount: > 400 ug
- Compatible lysis buffer: mild, non-denaturing lysis, e.g., RIPA, T-per, M-per (avoid high salt/detergent concentrations)
- Samples are stored at −80°C prior to shipping
- Assay Service Guide (Includes detailed sample preparation and submission instructions and forms)
- Samples must be frozen and shipped on dry ice in an insulated container
- Include sufficient dry ice for 48+ hours of transit time. Arrange shipment to ship early in the week (Monday–Wednesday for domestic shipments; Monday for international shipments) via overnight or international priority service.
- Email package tracking number to moc.o1773434058ibnoo1773434058mlluf1773434058@trop1773434058pus1773434058 to notify us of incoming shipments.
- Documentation
- A complete Sample Submission Form (MS WORD) placed inside your shipping container.
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If submitting from outside the U.S., include required customs documents:
- Commercial invoice
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Shipper’s Declaration stating “Non-hazardous biological sample for research use only”. This declaration must be completed on your institution’s letterhead.
Price: $1,780/sample
Note: The price listed is per sample per condition. Submitting both a control and a treatment sample counts as two samples.
U.S. Customers
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Online Orders: credit card only
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Purchase Orders (POs): We accept institutional or corporate POs. Email your PO to moc.o1773434058ibnoo1773434058mlluf1773434058@sred1773434058ro1773434058.
International Customers
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Prepayment Required: Payment must be made in advance by credit card or wire transfer.
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Please contact us for a formal quote, invoice, and payment instructions.
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We’ll guide you through the sample shipping process and required documentation.
