AKT Pathway Phospho Antibody Array

AKT Pathway Phospho Antibody Array

AKT Pathway Phospho Antibody Array features 137 highly specific antibodies related to the AKT pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog #: PAA137

$500.00

AKT Pathway Phospho Antibody Array

AKT Pathway Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. This array is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. It features site-specific and phospho-specific antibodies, allowing researchers to study serine, threonine, and tyrosine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening
  • Suitable samples include: cell lysates; frozen or FFPE tissue lysates
  • Glass based antibody array with high specificity and low background
  • Antibodies covalently attached to 3D polymer coated glass surface
  • Sensitive fluorescent detection
User’s Guide  

pdf    Antibody Array User’s Guide

Product Details
Number of Antibodies: 137 site-specific and phospho-specific antibodies
Number of Replicates: 6 replicates per antibody
Reactivity: Human: 100%; Mouse: 83%; Rat: 69%
Detailed Reactivity List
Internal Controls: Positive controls: beta-actin; GAPDH
Negative controls
Detection Method: Fluorescence
Compatible Scanners
Slide dimensions: 76 x 25 x 1 mm
Spot diameter: 260 – 280 um
Size: 2 array slides per package for analyzing two samples (control vs. treated)
Storage Condition: 4°C for 6 months
Recent Publications

Lida M, Brand, TM, Targeting AKT with the allosteric AKT inhibitor MK-2206 in non-small cell lung cancer cells with acquired resistance to cetuximab, Cancer Biology & Therapy 2013; 14(6),p. 481

Zhong D, Liu X, LKB1 Is Necessary for Akt-Mediated Phosphorylation of Proapoptotic Proteins, Cancer Research 2008; 68:7270-7277

Ordering Information 
Catalog No Description Size Price
PAA137 AKT Pathway Phospho Antibody Array 2 slides/pk $500
Additional Reagent Needed:

The ELISA based antibody array platform involves four major steps: 1) Protein extraction with non-denaturing lysis buffer; 2) Biotinylation of protein samples; 3) Incubation of labeled samples with antibody array; and 4) Detection by dye conjugated streptavidin.
Antibody Array

  Questions? Visit our Frequently Asked Questions page.

4E-BP1 (Thr36), 4E-BP1 (Thr45), AFX (Ser197), AKT (Thr308), AKT (Ser473), AKT2 (Ser474), ASK1 (Ser83), ASK1 (Ser966), BAD (Ser112), BAD (Ser136), BAD (Ser155), BCL-2 (Thr56), BCL-2 (Ser70), BCL-XL (Ser62), c-Jun (Ser63), c-Jun (Ser73), c-Jun (Ser243), c-Jun (Thr91), c-Jun (Thr93), c-Jun (Thr239), c-Jun (Tyr170), c-Kit (Tyr721), eIF4E (Ser209), FAK (Tyr397), FAK (Tyr861), FAK (Tyr925), FKHR (Ser256), FKHR (Ser319), FKHRL1 (Ser253), GAB1 (Tyr627), GSK3 alpha (Ser21), GSK3 beta (Ser9), IKB alpha (Ser32/36), IKB alpha (Ser42), IKK alpha (Thr23), IRS1 (Ser307), IRS1 (Ser312), IRS1 (Ser636), IRS1 (Ser639), JAK1 (Tyr1022), JAK2 (Tyr221), JAK2 (Tyr1007), Met (Tyr1234), Met (Tyr1349), p21Cip1 (Thr145), p27Kip1 (Ser10), p27Kip1 (Thr187), p53 (Ser6), p53 (Ser9), p53 (Ser15), p53 (Ser18), p53( Ser33), p53 (Ser37), p53 (Ser46), p53 (Ser315), p53 (Thr18), p70S6K (Ser411), p70S6K (Ser424), Paxillin (Tyr31), Paxillin (Tyr118), PDK1 (Ser241), PTEN (Ser370), PTEN (Ser380), PTEN (Ser380/Thr382/Thr383), Pyk2 (Tyr402), Raf1 (Ser259), Raf1 (Ser338), S6 Ribosomal Protein (Ser235), Zap70 (Tyr319), Zap70 (Tyr493)

pdf    Antibody Array User’s Guide

excel    Antibody List

excel    Array Map

gal    GAL File (To download, right click on the file name, then choose “Save target as”)

pdf    Material Safety Data Sheet (MSDS)

Lida M, Brand, TM, Targeting AKT with the allosteric AKT inhibitor MK-2206 in non-small cell lung cancer cells with acquired resistance to cetuximab, Cancer Biology & Therapy 2013; 14(6),p. 481

Zhong D, Liu X, LKB1 Is Necessary for Akt-Mediated Phosphorylation of Proapoptotic Proteins, Cancer Research 2008; 68:7270-7277

Additional Publications

Complete Antibody Array Assay Service

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,200 per sample

Array Scanning Service

Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. This service is free of charge.

Array Image Analysis Service

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $200 per slide

 

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