VEGF Pathway Phospho Antibody Array

VEGF Pathway Phospho Antibody Array

VEGF Antibody Array features 185 highly specific antibodies related to the VEGF pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples in human, mouse, or rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog No: PVE185

$670.00

VEGF Pathway Phospho Antibody Array

VEGF Pathway Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine phosphorylation and serine/threonine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening with 185 antibodies
  • Antibodies covalently immobilized on 3D polymer coated glass slide
  • Fluorescent detection
Specifications
Product Size:2 array slides per package for two samples (untreated vs. treated)
Reactivity:Human: 100% | Mouse: 95% |Rat: 84%
Suitable Sample Type:Cell lysate | Tissue lysate
Detection Method:Fluorescence | Compatible Scanners
Internal Controls:Positive controls: beta-actin | GAPDH | Negative controls
Slide dimensions: 76 x 25 x 1 mm
Storage Condition:4°C for 6 months

 

PRODUCT DETAILS

AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Thr450), AKT1 (Thr72), AKT2 (Ser474), BAD (Ser112), BAD (Ser134), BAD (Ser136), BAD (Ser155), BAD (Ser91/128), Casp9 (Ser144), Casp9 (Ser196), Casp9 (Tyr153), c-PLA2 (Ser505), Elk1 (Ser383), Elk1 (Ser389), Elk1 (Thr417), eNOS (Ser1177), eNOS (Ser615), eNOS (Thr495), ERK1/2, ERK3 (Ser189), ERK8 (Thr175/Tyr177), FAK (Ser910), FAK (Tyr397), FAK (Tyr407), FAK (Tyr576), FAK (Tyr861), FAK (Tyr925), FKHR (Ser256), FKHR (Ser319), GRB2 (Ser159), GTPase activating protein (Phopspho-Ser387), HSP27 (Ser15), HSP27 (Ser78), HSP27 (Ser82), HSP90 co-chaperone Cdc37 (Ser13), HSP90A, HSP90B (Ser254), IP3KA, IP3KC, MAPKAPK2 (Ser272), MAPKAPK2 (Thr222), MAPKAPK2 (Thr334), MEK1 (Ser217), MEK1 (Ser221), MEK1 (Ser298), MEK1 (Thr286), MEK1 (Thr291), MEK2 (Thr394), MKK3 (Ser189), MKK3 (Thr222), MKK6 (Ser207), P38 MAPK (Thr180), P38 MAPK (Tyr182), P38 MAPK (Tyr322), p44/42 MAPK (Thr202), p44/42 MAPK (Tyr204), Paxillin (Tyr118), Paxillin (Tyr31), PI3K-p85-a (Tyr607), PI3K-p85-a/g (Tyr467/Tyr199), PKC-a (Tyr657), PKC a/b II (Thr638), PKC-b (Ser661), PKC delta (Ser645), PKC delta (Thr505), PKC epsilon (Ser729), PKC theta (Ser676), PKC theta (Thr538), PKC zeta (Ab-410), PKC zeta (Ab-560), PKC zeta (Thr410), PKC zeta (Thr560), PLC-g1 (Tyr1253), PLC-g1 (Tyr771), PLC-g1 (Tyr783), PLC-g2 (Tyr1217), PLC-g2 (Tyr753), Raf1 (Phospho-Ser621), Raf1 (Ser259), Raf1 (Ser338), Raf1 (Tyr341), RapGEF1 (Tyr504), Ras-GRF1 (Ser916), Shc (Tyr349), Shc (Tyr427), Src (Ser75), Src (Tyr216), Src (Tyr418), Src (Tyr529), VEGFR1 (Tyr1333), VEGFR2 (Tyr1054), VEGFR2 (Tyr1059), VEGFR2 (Tyr1175), VEGFR2 (Tyr1214), VEGFR2 (Tyr951)

The ELISA based VEGF Pathway Phospho Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Antibody Array - How It Works

Antibody Array User’s Guide

Antibody List

Array Map

GAL File  (To download, right click on the file name, then choose “Save target as”)

Material Safety Data Sheet (MSDS)

Frequently Asked Questions

ADDITIONAL SERVICES

If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.

Cost: Free

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $200 per slide

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,470 per sample

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