AKT/PKB Phospho Antibody Array

AKT/PKB Phospho Antibody Array

AKT/PKB Phospho Antibody Array features 216 highly specific antibodies related to the AKT/PKB pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples from human, mouse, and rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Catalog No: PAB216

$730.00

AKT/PKB Phospho Antibody Array

The Phospho AKT/PKB Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.  This array features site-specific and phospho-specific antibodies, allowing researchers to study serine, threonine, and tyrosine phosphorylation at specific sites.

Key Features
  • Site-specific phosphorylation profiling and screening with 216 antibodies
  • Antibodies covalently immobilized on 3D polymer coated glass slide
  • Fluorescent detection
Specifications
Product Size:2 array slides per package for analyzing two samples (control vs. treated)
Featured Antibodies:216 site-specific and phospho-specific antibodies
Reactivity:Human: 100% | Mouse: 90% | Rat: 78%
Internal Controls:Positive controls: beta-actin; GAPDH ~~Negative controls
Suitable Sample Type:Cell lysate | Tissue lysate
Detection Method:Fluorescence; Compatible Scanners
Internal Controls:beta-actin | GAPDH | Negative controls
Slide Dimensions: 76 x 25 x 1 mm
Storage Condition:4°C for 6 months
PRODUCT DETAILS

14-3-3 theta/tau (Ser232), 14-3-3 zeta (Ser58), 14-3-3 zeta/delta (Thr232), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT1S1 (Thr246), AKT2 (Ser474), BAD (Ser112), BAD (Ser134), BAD (Ser136), BAD (Ser155), BAD (Ser91/128), BCL-2 (Ser70), BCL-2 (Ser87), BCL-2 (Thr56), BCL-2 (Thr69), BIM (Ser69/65), Cyclin D1 (Thr286), eNOS (Ser1177), eNOS (Ser615), eNOS (Thr495), FAK (Ser910), FAK (Tyr397), FAK (Tyr407), FAK (Tyr576), FAK (Tyr861), FAK (Tyr925), FKHR (Ser256), FKHR (Ser319), FOXO1/3/4-PAN (Thr24/32), FOXO1A (Ser329), FOXO1A/3A (Ser322/325), Gab1 (Tyr627), Gab1 (Tyr659), Gab2 (Tyr643), GABA-RB (Ser434), GSK3a-b (Tyr216/279), GSK3a (Ser21), GSK3b (Ser9), IKKa (Thr23), IKKa/b (Ser180/181), IRS-1 (Ser1101), IRS-1 (Ser307), IRS-1 (Ser312), IRS-1 (Ser323), IRS-1 (Ser612), IRS-1 (Ser636), IRS-1 (Ser639), IRS-1 (Ser794), JAK1 (Tyr1022), LYN (Tyr507), mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), MYT1, p21Cip1 (Thr145), p27Kip1 (Ser10), p27Kip1 (Thr187), p53 (Ser6), p53 (Ser9), p53 (Ser15), p53 (Ser20), p53 (Ser33), p53 (Ser37), p53 (Ser46), p53 (Ser315), p53 (Ser366), p53 (Ser378), p53 (Ser392), p53 (Thr18), p53 (Thr81), p70S6K (Ser371), p70S6K (Ser411), p70S6K (Ser418), p70S6K (Ser424), p70S6K (Thr229), p70S6K (Thr389), p70S6K (Thr421), p70S6Kb (Ser423), Paxillin (Ab-118), Paxillin (Ab-31), Paxillin (Tyr118), Paxillin (Tyr31), PDK1 (Ser241), PFKFB2 (Ser483), PI3K-p85-alpha (Tyr607), PI3K-p85-a/g (Tyr467/Tyr199), PIP5K (Ser307), PP2A-a (Tyr307), PTEN (Ser370), PTEN (Ser380), PTEN (Ser380/Thr382/Thr383), RapGEF1 (Tyr504), S6 Ribosomal Protein (Ser235), S6K, SYK (Tyr323), SYK (Tyr348), SYK (Tyr525), Synapsin1 (Ab-62), Synapsin1 (Ser62), Tuberin/TSC2 (Ser939), Tuberin/TSC2 (Thr1462), WEE1 (Ser642), XIAP (Ser87)

The ELISA based AKB/PKB Phospho Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Antibody Array - How It Works

Morande PE, Sivina M, Ibrutinib therapy downregulates AID enzyme and proliferative fractions in chronic lymphocytic leukemia, Blood 2019 :blood-2018-09-876292

Peppenelli M, Miller M, Aberrant regulation of the Akt signaling network by human cytomegalovirus allows for targeting of infected monocytesAntiviral Res, 2018 Jul 25. pii: S0166-3542(18)30211-0. doi: 10.1016/j.antiviral.2018.07.015

Reinhart R, Kaufmann T, IL-4 enhances survival of in vitro-differentiated mouse basophils through transcription-independent signaling downstream of PI3KCell Death & Disease, 2018 9:713

Shi F, Zhang J, The dual PI3K/mTOR inhibitor dactolisib elicits anti-tumor activity in vitro and in vivo, Oncotarget, 2018 Jan 2; 9(1): 706–717

ADDITIONAL SERVICES

If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.

Cost: Free

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $250 per slide

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $1,520 per sample

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