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Tyrosine Phosphorylation ProArray

Introduction

Each Tyrosine Phosphorylation ProArray includes 228 site-specific tyrosine phospho-antibodies; each has six replicates.  The phospho-specific antibodies bind to and detect the protein only when the specific tyrosine site is phosphorylated.  The antibodies are printed on standard-size coated glass microscope slides and can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.    

Key Features

• Site-specific tyrosine phosphorylation antibodies

• Microarray format for high throughput expression profiling in cells, tissues and body fluids

• 3-D polymer based glass substrates

• High specificity and affinity; low background

• Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

• Each antibody comes with six replicates for more reliable and consistent results

• GenePix^® Array List (GAL) files are provided for convenient and accurate data analysis

• Optimized Antibody Array Assay Kit provides optimized reagents and buffers for protein extraction, labeling, coupling and detection

Applications

• Protein expression profiling (Qualitative)

• Measure changes in phosphorylation status at tyrosine sites

• Compare profiles of normal, diseased or treated samples

• Identify candidate biomarkers

Specifications

Slide dimensions:	76 x 25 x 1 mm	Spot diameter:	260 - 280 um
Barcode label:	23 x 9 mm	Spot-to-spot distance:	600 um

Technical Information

Antibody Array User's Guide

Antibody List

Array Layout

GAL File

Frequently Asked Questions

Components

Antibody arrays (2)

Storage Conditions

4°C for 6 months

References

Zhong D, Liu X, et al.: LKB1 Is Necessary for Akt-Mediated Phosphorylation of Proapoptotic Proteins, Cancer Research 2008, 68: 7270-7277.

Ordering Information

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