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Insulin Receptor Phospho-Specific Antibody Microarray

Introduction

The Insulin Receptor Phospho Antibody Array consists of 219 highly specific and well-characterized phosphorylation antibodies in the insulin receptor pathway; each antibody is printed with six replicates.  The phospho-specific antibodies bind to and detect the protein only when phosphorylated at the specific site.  The non-phospho pairs of the phospho-specific antibodies are included in the array to allow comparison based on phosphorylation state.  The antibodies are printed on standard-size coated glass microscope slides and can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.    

Key Features

• Unique collection of phospho-specific antibodies and their non-phospho pairs

• Microarray format for high throughput expression profiling protein extracts from cells, frozen or FFPE tissues, or serum samples

• Each antibody is spotted with six replicates for more reliable and consistent results

• 3-D polymer based glass substrates

• High specificity and affinity; low background

• Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

• GenePix^® Array List (GAL) files are provided for convenient and accurate data analysis

• Antibody Array Assay Kit provides optimized reagents and buffers for protein extraction, labeling, conjugation and detection

Applications

• Protein expression profiling (Qualitative)

• Measure changes in phosphorylation status at specific sites

• Compare profiles of normal, diseased or treated samples

• Identify candidate biomarkers

Specifications

Slide dimensions:	76 x 25 x 1 mm	Spot diameter:	260 - 280 um
Barcode label:	23 x 9 mm	Spot-to-spot distance:	600 um

Technical Information

Antibody Array User's Guide

Antibody List

Array Layout

GAL File

Frequently Asked Questions

References

He H, Zong Y, et al. Sensing the Insulin Signaling Pathway with an Antibody Array, Proteomics Clinical Applications 2009;3:1440–1450.

Additional References

Components

Antibody arrays (2)

Storage Conditions

4°C for 6 months

Ordering Information

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