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Phospho Explorer Antibody Microarray

Introduction

The Phospho Explorer Antibody Array is a microarray consisting of 1318 well-characterized phospho-specific antibodies.  It is broad in scope and allows researchers to detect and analyze hundreds of proteins simultaneously on a single slide.  It is a great tool for biomarker screening and discovery.

The antibodies are printed on standard-size coated glass microscope slides and can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides.    

Key Features

• Unique collection of phospho-specific antibodies and their non-phospho pairs

• Microarray format for high throughput expression profiling protein extracts from cells, frozen or FFPE tissues, or serum samples

• Each antibody is spotted with two replicates for more reliable and consistent results

• 3-D polymer based glass substrates

• High specificity and affinity; low background

• Suitable for detection on all fluorescent microarray scanners compatible with 76x25x1mm slides

• GenePix^® Array List (GAL) files are provided for convenient and accurate data analysis

• Antibody Array Assay Kit provides optimized reagents and buffers for protein extraction, labeling, conjugation and detection

Applications

• Protein expression profiling (Qualitative)

• Measure changes in phosphorylation status at specific sites

• Compare profiles of normal, diseased or treated samples

• Identify candidate biomarkers

Specifications

Slide dimensions:	76 x 25 x 1 mm	Spot diameter:	220-250 um
Barcode label:	23 x 9 mm	Spot-to-spot distance:	450 um

Technical Information

Antibody Array User's Guide

Antibody List / Reactivity

Array Layout

Gal File

Frequently Asked Questions

Components

Antibody arrays (2)

Storage Conditions

4°C for 6 months

Selected References

Bernier M, Paul RK, et al.: Negative Regulation of STAT3-mediated Cellular Respiration by SirT1, Journal of Biological Chemistry 2011; JBC Papers in Press. Published on April 5, 2011 as Manuscript M110.200311

Fan H, Yang L, et al.: Cardioprotective effects of salvianolic Acid a on myocardial ischemia-reperfusion injury in vivo and in vitro, Evidence Based Complement Alternative Medicine 2012, 2012:508938

Hu Y, Pioli PD, Siegel E, Zhang Q, Nelson J, Chaturbedi A, Mathews MS, Ro DI, Alkafeef S, Hsu N, Hamamura M, Yu L, Hess KR, Tromberg BJ, Linskey ME, Zhou YH, EFEMP1 suppresses malignant glioma growth and exerts its action within the tumor extracellular compartment, Molecular Cancer 2011, 10:123

Kang S, Elf S, et al.: p90 Ribosomal S6 Kinase 2 Promotes Invasion and Metastasis of Human Head and Neck Squamous Cell Carcinoma Cells, Journal of Clinical Investigation 2010, 120(4):1165-1177

Yu Y, Richardson D: Cellular iron depletion stimulates the JNK and p38 MAPK signaling transduction pathways, dissociation of ASK1-thioredoxin, and activation of ASK1, Journal of Biological Chemistry 2011, 286(17):15413-27

Additional references

Ordering Information

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